Budo Camille, Goffinet G, Bellotto Dennis, Petroll W Matthew
Laboratory of Ultrastructural Morphology, Zoological Institute, University of Liège, Liège, Belgium.
J Cataract Refract Surg. 2003 Dec;29(12):2411-8. doi: 10.1016/s0886-3350(03)00410-3.
To investigate the morphological effects of Viscoat (sodium hyaluronate 3.0%-chondroitin sulfate 4.0%) on lens epithelial cells (LECs).
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas, USA, and the Laboratory of Ultrastructural Morphology, Zoological Institute, University of Liège, Liège, Belgium.
Human LECs collected via capsulorhexis were examined by light microscopy (LM) and transmission electron microscopy (TEM). Lens epithelial cells from rabbit capsulorhexis samples were studied by LM and TEM following exposure to Provisc (sodium hyaluronate 1.0%) or Viscoat ophthalmic viscoelastic device (OVD). Since Viscoat is hypertonic (340 mOsm), hypertonic, isotonic, and hypotonic solutions were compared to investigate a possible mechanism for the observed effects. The effects of Provisc and Viscoat on rabbit LECs in the intact lens were also compared.
Human LECs gathered via capsulorhexis following exposure to Viscoat were generally thinner than control samples and often had condensed nuclei and increased intracellular vacuolization. Rabbit capsular tissue exposed in situ to Viscoat demonstrated changes similar to those seen in humans. Cells exposed to Provisc were similar to cells in untreated controls in humans and rabbits. Corneal endothelial cells exposed to either agent were unaffected. Experiments with hypertonic and hypotonic buffers induced some of the changes noted with Viscoat, but the effects were less severe. Lens epithelial cells in intact rabbit lenses exposed to Viscoat appeared similar to LECs in the control samples.
Light microscopy and TEM of human lens capsule tissue suggest that Viscoat induces significant morphological changes in LECs during cataract surgery. The changes may underlie the improved visualization of these cells that has been reported during cataract surgery. Corneal endothelial cells were unaffected by exposure to Viscoat. Studies in a rabbit model suggest that the hyperosmolarity of Viscoat may play a partial role in the LEC changes.
研究Viscoat（3.0%透明质酸钠-4.0%硫酸软骨素）对晶状体上皮细胞（LECs）的形态学影响。
美国得克萨斯州达拉斯市德克萨斯大学西南医学中心眼科以及比利时列日大学动物学研究所超微结构形态学实验室。
通过撕囊收集的人LECs采用光学显微镜（LM）和透射电子显微镜（TEM）进行检查。兔撕囊样本的晶状体上皮细胞在暴露于Provisc（1.0%透明质酸钠）或Viscoat眼科粘弹剂（OVD）后，采用LM和TEM进行研究。由于Viscoat是高渗的（340 mOsm），因此比较了高渗、等渗和低渗溶液，以研究观察到的效应的可能机制。还比较了Provisc和Viscoat对完整晶状体中兔LECs的影响。
暴露于Viscoat后通过撕囊收集的人LECs通常比对照样本更薄，细胞核常浓缩，细胞内空泡化增加。原位暴露于Viscoat的兔囊膜组织表现出与人类相似的变化。暴露于Provisc的细胞与人类和兔未处理对照中的细胞相似。暴露于任何一种制剂的角膜内皮细胞均未受影响。用高渗和低渗缓冲液进行的实验诱导了一些与Viscoat相关的变化，但效应较轻。暴露于Viscoat的完整兔晶状体中的晶状体上皮细胞与对照样本中的LECs相似。
人晶状体囊膜组织的光学显微镜和TEM检查表明，Viscoat在白内障手术期间可诱导LECs发生显著的形态学变化。这些变化可能是白内障手术期间报告的这些细胞可视化改善的基础。暴露于Viscoat对角膜内皮细胞无影响。兔模型研究表明，Viscoat的高渗性可能在LECs变化中起部分作用。
