Yao Hongwei, Wan Yuanlian, Ye Jingming, Wu Nan, Ju Xiaoming, Zhu Jing
Department of General Surgery Peking University First Hospital Beijing 100034 China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Dec 18;35(6):634-8.
To analyze the role of tissue factor (TF) in the in vitro invasive ability of human colon carcinoma cells (HT-29).
The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. In vitro Matrigel invasion assays were used to show the invasive ability of HT-29 cells with sense/antisense TFcDNA transfection.
HT-29 cells with sense-TFcDNA transfection showed increased TF expression and invasive ability compared with the cells without transfection, but HT-29 cells with antisense-TFcDNA transfection got the contrary change.
TF can increase the invasive ability of HT-29 cells in vitro.
分析组织因子（TF）在人结肠癌细胞（HT-29）体外侵袭能力中的作用。
采用脂质体2000法将携带正义或反义TFcDNA的真核表达载体pcDNA3.1/Zeo转染至HT-29细胞。通过蛋白质免疫印迹法检测转染细胞中的TF蛋白。采用体外基质胶侵袭实验检测转染正义/反义TFcDNA的HT-29细胞的侵袭能力。
与未转染细胞相比，转染正义TFcDNA的HT-29细胞TF表达增加且侵袭能力增强，但转染反义TFcDNA的HT-29细胞出现相反变化。
TF可增强HT-29细胞的体外侵袭能力。
