Wan Yuan-lian, Yao Hong-wei, Ye Jing-ming, Liu Yu-cun, Wu Tao, Wang Xin, Pan Yi-sheng, Wu Nan, Ju Xiao-ming, Zhu Jing, Huang Yan-ting
Department of General Surgery, The First Hospital of Peking University, Beijing 100034, China.
Zhonghua Wai Ke Za Zhi. 2004 Feb 7;42(3):149-53.
To investigate the role of tissue factor (TF) expression in the invasive and metastatic ability of colorectal carcinoma and explore the influence of TF on the invasive ability of HT-29 cells.
TF expression of specimens from 85 colorectal carcinomas and 6 colorectal adenomas was observed by immunohistochemistry. The role of TF expression in prognosis and tumor invasion and metastasis was analyzed. The plasmids pcDNA3.1/Zeo bearing either sense or antisense-TFcDNA were transfected into HT-29 cells by the way of Lipofectamine 2000. TF proteins in transfected and untransfected HT-29cells were detected by Western blot. In vitro Matrigel invasion assays were performed to show the invasive ability of those cells.
TF expression was positive in 40 (47.1%) of 85 colorectal carcinoma specimens, but negative in normal mucosa and adenoma specimens. TF expression showed significant correlation with tumor invasive depth (r = 0.895, P < 0.01). TF expression showed significant correlation with synchronous and metachronous hepatic metastasis (r = 0.974, P < 0.01 and r = 0.963, P < 0.01 respectively). TF expression was a significant risk factor for hepatic metastasis (P < 0.01) and prognosis (P < 0.01). TF expression in HT-29 cells with sense/antisense-TFcDNA transfection was more/less than that of the cells without transfection. The invasive ability of HT-29 cells with sense-TFcDNA transfection was increased in vitro compared with the untransfected cells, but HT-29 cells with antisense-TFcDNA transfection got the contrary change.
TF may take part in the invasive and metastatic process of primary colorectal carcinoma, and TF expression may be an indicator of hepatic metastasis and prognosis for colorectal carcinoma patients. TF expression may increase the invasive ability of HT-29 cell in vitro.
探讨组织因子（TF）表达在结直肠癌侵袭和转移能力中的作用，并探究TF对HT-29细胞侵袭能力的影响。
采用免疫组织化学法观察85例结直肠癌标本和6例结直肠腺瘤标本中TF的表达情况。分析TF表达在预后及肿瘤侵袭和转移中的作用。通过脂质体2000将携带正义或反义TFcDNA的质粒pcDNA3.1/Zeo转染至HT-29细胞。采用蛋白质免疫印迹法检测转染和未转染的HT-29细胞中的TF蛋白。进行体外基质胶侵袭实验以显示这些细胞的侵袭能力。
85例结直肠癌标本中有40例（47.1%）TF表达呈阳性，而在正常黏膜和腺瘤标本中呈阴性。TF表达与肿瘤浸润深度显著相关（r = 0.895，P < 0.01）。TF表达与同时性和异时性肝转移显著相关（分别为r = 0.974，P < 0.01和r = 0.963，P < 0.01）。TF表达是肝转移（P < 0.01）和预后（P < 0.01）的显著危险因素。正义/反义TFcDNA转染的HT-29细胞中TF表达高于/低于未转染细胞。与未转染细胞相比，正义TFcDNA转染的HT-29细胞体外侵袭能力增强，而反义TFcDNA转染的HT-29细胞则出现相反变化。
TF可能参与原发性结直肠癌的侵袭和转移过程，TF表达可能是结直肠癌患者肝转移和预后的一个指标。TF表达可能增强HT-29细胞的体外侵袭能力。
