Dong Xiuyun, Zhou Liya, Lin Sanren, Ru Binggen, Fang Min
Department of Gastroenterology, Peking University Third Hospital, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Dec 18;35(6):639-41.
To study the effect of recombinant human intestinal trefoil factor ( rhITF) on the healing of rat chronic gastric ulcer, protect gastric mucosal and mechanisms are involved.
(1) Acute gastric mucosal injury was induced by ethanol, stress, aspirin and pylorusl ligation. The injury index,MDA, GMBL,hexosamine (Hex) and acid output were measure. (2) Chronic gastric ulcer was induced in rats by application of 50% glacial acetic acid to the serosa of the glandular stomach. After injury, rats received by rhITF or vehicle orally twice daily for 11 days. On day 12, gastric mucosal blood flow GMBF was measured under ether anesthesia. Then the pylorus was ligated for 3 hours and each stomach removed. The gastric acid output, ulcer index, Hex and nitric oxide(NO) content in gastric mucosa, as well as iNOSmRNA in the ulcer bed were determined.
(1) rhITF protected gastric mucosa from the acute lesion, and increased Hex content in gastric mucosa. (2) rhITF treatment significantly decreased the ulcer index and gastric acid output, but increased the GMBF, Hex and NO content in comparison with the control groups. In addition, rhITF also stimulated iNOSmRNA expression in the ulcer bed by situ hybridization analysis.
rhITF can protect gastric mucosa against acute lesion, and enhance the healing of chronic gastric ulcer in the rats. This action may result from the inhibition of gastric acid output, increase of GMBF.Hex and NO content and rhITF stimulated iNOSmRNA expression.
研究重组人肠三叶因子（rhITF）对大鼠慢性胃溃疡愈合的影响、保护胃黏膜及其作用机制。
（1）采用乙醇、应激、阿司匹林和幽门结扎法诱导急性胃黏膜损伤。测定损伤指数、丙二醛（MDA）、胃黏液（GMBL）、氨基己糖（Hex）和胃酸分泌量。（2）用50%冰醋酸涂抹大鼠腺胃浆膜诱导慢性胃溃疡。造模后，大鼠每日口服rhITF或赋形剂2次，共11天。第12天，在乙醚麻醉下测定胃黏膜血流量（GMBF）。然后结扎幽门3小时，取出每只大鼠的胃。测定胃酸分泌量、溃疡指数、胃黏膜中Hex和一氧化氮（NO）含量，以及溃疡灶中诱导型一氧化氮合酶（iNOS）mRNA水平。
（1）rhITF可保护胃黏膜免受急性损伤，并增加胃黏膜中Hex含量。（2）与对照组相比，rhITF治疗可显著降低溃疡指数和胃酸分泌量，但增加GMBF、Hex和NO含量。此外，原位杂交分析显示rhITF还可刺激溃疡灶中iNOSmRNA表达。
rhITF可保护胃黏膜免受急性损伤，促进大鼠慢性胃溃疡愈合。其作用机制可能是抑制胃酸分泌、增加GMBF、Hex和NO含量，以及刺激iNOSmRNA表达。
