Kang M S, Oh M J, Kim Y J, Kawai K, Jung S J
Department of Fish Pathology, Yosu National University, Yosu, Chunnam, Republic of Korea.
J Fish Dis. 2003 Nov-Dec;26(11-12):657-65. doi: 10.1046/j.1365-2761.2003.00499.x.
Two new cell cultures from flounder, Paralichthys olivaceus (Temminck & Schlegel), flounder fin (FFN) cells from fin tissue and flounder spleen (FSP) cells from spleen tissue, were established and characterized. The cells multiplied well in Eagle's minimum essential medium, supplemented with 10% foetal bovine serum, and have been subcultured more than 100 times, becoming continuous cell lines. Modal diploid chromosome number of FFN and FSP cells was 64 and 62, respectively. Polymerase chain reaction products were obtained from FFN and FSP cells with primer sets ofmicrosatellite markers of flounder. Optimal growth temperature was 20 degrees C and consisted of epithelioid cells. FFN and FSP cells showed cytopathic effects after inoculation of infectious pancreatic necrosis virus, marine birnavirus, chum salmon virus, infectious haematopoietic necrosis virus, spring viraemia of carp virus and hirame rhabdovirus. Thus these new cell lines may be useful for studying a wide range of fish viruses.
建立并鉴定了两种新的牙鲆（Paralichthys olivaceus (Temminck & Schlegel)）细胞培养物，即来自鳍组织的牙鲆鳍（FFN）细胞和来自脾组织的牙鲆脾（FSP）细胞。这些细胞在补充有10%胎牛血清的Eagle's最低必需培养基中生长良好，并且已经传代培养超过100次，成为连续细胞系。FFN和FSP细胞的模式二倍体染色体数分别为64和62。使用牙鲆微卫星标记引物对从FFN和FSP细胞中获得了聚合酶链反应产物。最佳生长温度为20℃，由上皮样细胞组成。接种传染性胰腺坏死病毒、海洋双RNA病毒、大麻哈鱼病毒、传染性造血坏死病毒、鲤春病毒血症病毒和牙鲆弹状病毒后，FFN和FSP细胞出现细胞病变效应。因此，这些新的细胞系可能有助于研究多种鱼类病毒。
