Sistig Suzana, Lukac J, Vucicevic-Boras V, Delic D, Kusic Z
Department of Oral Medicine, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
Eur J Med Res. 2003 Dec 9;8(12):543-8.
Many of the opportunistic infections in HIV are related to the mucous membranes, but the protection of oral mucosal compartment mediated by IgA and IgG subclasses in saliva is still not well documented. Therefore, the aim of this study was to investigate the status of salivary IgA and IgG subclass in HIV positive patients. Levels of IgG1, IgG2, IgG3 and IgG4 were determined by ELISA and IgA1 and IgA2 by radial immunodiffusion in the resting whole saliva of 33 HIV patients and 21 HIV-seronegative healthy controls and were expressed in mg/L (IgA) and ug/L (IgG) of saliva. Salivary IgA2 was significantly reduced in the HIV positive patients (p<0.0009) when compared to the healthy controls, but no differences in salivary IgA1 level between HIV patients and healthy controls were found. On the other hand, salivary IgG1, IgG2, IgG3 and IgG4 subclasses were increased (p<0.0009) in HIV patients in comparison to the healthy controls. We can conclude that inspite of IgA2 deficiency which was found in studied HIV positive patients, mucosal antibody responses are quite normal and might not predispose development of oral opportunistic infections.
许多HIV相关的机会性感染都与黏膜有关，但唾液中由IgA和IgG亚类介导的口腔黏膜区保护作用仍未得到充分记录。因此，本研究的目的是调查HIV阳性患者唾液中IgA和IgG亚类的状况。通过ELISA法测定33例HIV患者和21例HIV血清阴性健康对照者静息全唾液中IgG1、IgG2、IgG3和IgG4的水平，通过放射免疫扩散法测定IgA1和IgA2的水平，并以唾液的mg/L（IgA）和μg/L（IgG）表示。与健康对照相比，HIV阳性患者的唾液IgA2显著降低（p<0.0009），但HIV患者与健康对照之间的唾液IgA1水平未发现差异。另一方面，与健康对照相比，HIV患者的唾液IgG1、IgG2、IgG3和IgG4亚类升高（p<0.0009）。我们可以得出结论，尽管在所研究的HIV阳性患者中发现了IgA2缺乏，但黏膜抗体反应相当正常，可能不会引发口腔机会性感染的发生。
