低温过量表达的高活性可溶性大肠杆菌σ70多肽的纯化 - Suppr

Purification of highly-active and soluble Escherichia coli sigma 70 polypeptide overproduced at low temperature.

作者信息

Zhi Huijun, Jin Ding Jun

机构信息

Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bldg. 37, Rm. 5144, Bethesda, Maryland 20892, USA.

出版信息

Methods Enzymol. 2003;370:174-80. doi: 10.1016/S0076-6879(03)70015-9.

DOI:10.1016/S0076-6879(03)70015-9

PMID:14712643

Abstract

摘要

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Purification of highly-active and soluble Escherichia coli sigma 70 polypeptide overproduced at low temperature.

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