细菌和酵母RNA聚合酶延伸复合物的工程改造 - Suppr

Engineering of elongation complexes of bacterial and yeast RNA polymerases.

作者信息

Komissarova Natalia, Kireeva Maria L, Becker Jodi, Sidorenkov Igor, Kashlev Mikhail

机构信息

NCI Center for Cancer Research, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA.

出版信息

Methods Enzymol. 2003;371:233-51. doi: 10.1016/S0076-6879(03)71017-9.

DOI:10.1016/S0076-6879(03)71017-9

PMID:14712704

Abstract

摘要

相似文献

Engineering of elongation complexes of bacterial and yeast RNA polymerases.细菌和酵母RNA聚合酶延伸复合物的工程改造

Methods Enzymol. 2003;371:233-51. doi: 10.1016/S0076-6879(03)71017-9.

Structural analysis of ternary complexes of Escherichia coli RNA polymerase: ribonuclease footprinting of the nascent RNA in complexes.大肠杆菌RNA聚合酶三元复合物的结构分析：复合物中新生RNA的核糖核酸酶足迹分析

Biochemistry. 1999 Jan 5;38(1):218-25. doi: 10.1021/bi9818422.

Assay of intrinsic transcript termination by E. coli RNA polymerase on single-stranded and double-stranded DNA templates.大肠杆菌RNA聚合酶在单链和双链DNA模板上进行内在转录终止的测定。

Methods Enzymol. 2003;371:339-51. doi: 10.1016/S0076-6879(03)71025-8.

Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexes.

Methods Enzymol. 2003;370:553-67. doi: 10.1016/S0076-6879(03)70047-0.

Assay of antitermination of ribosomal RNA transcription.核糖体RNA转录抗终止测定

Methods Enzymol. 2003;371:472-87. doi: 10.1016/S0076-6879(03)71035-0.

Single DNA molecule analysis of transcription complexes.转录复合物的单DNA分子分析

Methods Enzymol. 2003;371:34-50. doi: 10.1016/S0076-6879(03)71003-9.

Rapid quench mixing to quantify kinetics of steps in association of Escherichia coli RNA polymerase with promoter DNA.快速淬灭混合法用于定量大肠杆菌RNA聚合酶与启动子DNA结合过程中各步骤的动力学。

Methods Enzymol. 2003;370:535-46. doi: 10.1016/S0076-6879(03)70045-7.

Using a lac repressor roadblock to analyze the E. coli transcription elongation complex.利用乳糖阻遏蛋白阻碍物分析大肠杆菌转录延伸复合物。

Methods Enzymol. 2003;371:207-18. doi: 10.1016/S0076-6879(03)71015-5.

Crystallographic analysis of Thermus aquaticus RNA polymerase holoenzyme and a holoenzyme/promoter DNA complex.嗜热水生栖热菌RNA聚合酶全酶及全酶/启动子DNA复合物的晶体学分析。

Methods Enzymol. 2003;370:42-53. doi: 10.1016/S0076-6879(03)70004-4.

Kinetics of multisubunit RNA polymerases: experimental methods and data analysis.多亚基RNA聚合酶的动力学：实验方法与数据分析

Methods Enzymol. 2003;371:71-81. doi: 10.1016/S0076-6879(03)71005-2.

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NDF/GLYR1 Promotes RNA Polymerase II Processivity via Pol II Binding and Nucleosome Destabilization.NDF/GLYR1通过与RNA聚合酶II结合和破坏核小体来促进RNA聚合酶II的持续合成能力。

Int J Mol Sci. 2025 May 19;26(10):4874. doi: 10.3390/ijms26104874.

Isolation of E. coli RNA polymerase transcription elongation complexes by selective solid-phase photoreversible immobilization.通过选择性固相光可逆固定化分离 E. coli RNA 聚合酶转录延伸复合物。

Methods Enzymol. 2023;691:223-250. doi: 10.1016/bs.mie.2023.03.019. Epub 2023 Apr 22.

A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery.尾随核糖体通过力和变构作用提高 RNA 聚合酶的速度，但其代价是转录保真度。

Cell. 2023 Mar 16;186(6):1244-1262.e34. doi: 10.1016/j.cell.2023.02.008.

An NTP-driven mechanism for the nucleotide addition cycle of Escherichia coli RNA polymerase during transcription.大肠杆菌 RNA 聚合酶转录过程中核苷酸添加循环的 NTP 驱动机制。

PLoS One. 2022 Oct 25;17(10):e0273746. doi: 10.1371/journal.pone.0273746. eCollection 2022.

Mechanism of transcription modulation by the transcription-repair coupling factor.转录修复偶联因子对转录调控的作用机制。

Nucleic Acids Res. 2022 Jun 10;50(10):5688-5712. doi: 10.1093/nar/gkac449.

Mutations compensating for the fitness cost of rifampicin resistance in Escherichia coli exert pleiotropic effect on RNA polymerase catalysis.突变补偿了大肠杆菌利福平耐药性的适应性成本，对 RNA 聚合酶的催化具有多效性影响。

Nucleic Acids Res. 2022 Jun 10;50(10):5739-5756. doi: 10.1093/nar/gkac406.

NDF is a transcription factor that stimulates elongation by RNA polymerase II.NDF 是一种转录因子，可刺激 RNA 聚合酶 II 的延伸。

Genes Dev. 2022 Mar 1;36(5-6):294-299. doi: 10.1101/gad.349150.121. Epub 2022 Mar 10.

Monitoring RNA dynamics in native transcriptional complexes.监测天然转录复合物中的 RNA 动态。

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Conserved Trigger Loop Histidine of RNA Polymerase II Functions as a Positional Catalyst Primarily through Steric Effects.保守的 RNA 聚合酶 II 触发环组氨酸主要通过空间位阻效应充当定位催化剂。

Biochemistry. 2021 Nov 9;60(44):3323-3336. doi: 10.1021/acs.biochem.1c00528. Epub 2021 Oct 27.

The mechanism of the nucleo-sugar selection by multi-subunit RNA polymerases.多亚基 RNA 聚合酶对核糖类的选择机制。

Nat Commun. 2021 Feb 4;12(1):796. doi: 10.1038/s41467-021-21005-w.

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Engineering of elongation complexes of bacterial and yeast RNA polymerases.

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