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[苏联用于检测HIV抗体的免疫酶测试系统中使用的过氧化物酶结合物的比较特征]

[The comparative characteristics of the peroxidase conjugates used in Soviet immunoenzyme test systems for detecting HIV antibodies].

作者信息

Vorob'eva M S, Bektimirov T A, Borsuk E A, Cheverdiuk L N, Vaneeva L I, Shkurina E A, Kanev A N

出版信息

Vopr Virusol. 1992 Jul-Aug;37(4):184-7.

PMID:1471340
Abstract

Five lots of peroxidase conjugates were studied in enzyme immunoassay systems "Recombinant-HIV" and "Peptoscreen-2" for detection of antibody to HIV. The conjugates differed from each other by the source of generation and methods of preparation. The conjugates were studied in biochemical tests and ELISA. When panels of sera from HIV-infected children and adults were employed, the advantages of using the anti-immunoglobulin conjugate over protein A-peroxidase conjugate were demonstrated, as the former increased the sensitivity of the test systems to HIV antibody detection.

摘要

在用于检测HIV抗体的“重组HIV”和“肽筛-2”酶免疫分析系统中,对五批过氧化物酶结合物进行了研究。这些结合物在产生来源和制备方法上彼此不同。对这些结合物进行了生化试验和酶联免疫吸附测定(ELISA)。当使用来自HIV感染儿童和成人的血清组时,证明了使用抗免疫球蛋白结合物比蛋白A-过氧化物酶结合物具有优势,因为前者提高了检测系统对HIV抗体检测的灵敏度。

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