Schibler Laurent, Roig Anne, Mahé Marie-Françoise, Save Jean-Claude, Gautier Mathieu, Taourit Sead, Boichard Didier, Eggen André, Cribiu Edmond P
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas, France.
Genet Sel Evol. 2004 Jan-Feb;36(1):105-22. doi: 10.1186/1297-9686-36-1-105.
A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105,984 clones) and a part of the CHORI-240 BAC library (26,500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100,923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.
通过结合荧光双酶切指纹图谱和序列标签位点（STS）标记筛选，构建了第一代基于克隆的牛基因组物理图谱。BAC克隆选自法国国家农业研究院（Inra）的BAC文库（105,984个克隆）和CHORI-240 BAC文库的一部分（26,500个克隆）。利用总共1303个标记（451个微卫星、471个基因、127个EST和254个BAC末端）的筛选信息对重叠群进行定位。最终图谱由从100,923个克隆组装而成的6615个重叠群组成，将成为反刍动物基因组研究的宝贵工具，包括靶向标记生产、定位克隆或特定感兴趣区域的靶向测序。
