Yasumatsu Ryuji, Kuratomi Yuichiro, Nakashima Torahiko, Masuda Muneyuki, Yamamoto Tomoya
Department of Otorhinolaryngology, Graduate School of Medical Sciences of Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812-8582 Fukuoka, Japan.
Eur Arch Otorhinolaryngol. 2004 Nov;261(10):526-30. doi: 10.1007/s00405-003-0724-6. Epub 2004 Jan 9.
Adenoid cystic carcinoma is a malignant tumor of salivary gland origin. It tends to grow slowly, but shows frequent recurrence and metastasis. Cyclin D1, a cell-cycle regulation protein, has been reported to be overexpressed in various types of cancer and to correlate with poor survival of the patients. However, the prognostic significance of cyclin D1 expression in ACC of the salivary glands has not yet been determined. To evaluate the role of cyclin D1 in the biological regulation of ACC, we constitutively expressed an antisense cyclin D1 complementary DNA (cDNA) in an established ACC cell line that exhibits high endogenous expression of cyclin D1. The effect of cyclin D1 expression on in vitro cell growth and cell cycle were examined. In addition, we also examined the immunohistochemical expression of cyclin D1 protein in 31 cases of ACC of the salivary gland and correlated its expression with proliferative activity or prognosis. There were no significant differences of the in vitro growth and in the percentage of the total cell population in the G1 phase and S phase between antisense cyclin D1 clones and control clones. Thirty-two percent of tumors derived from surgical specimens examined were immunohistochemically positive for cyclin D1 protein. No association was found between cyclin D1 expression and cell proliferation or the clinical outcome of the patients. It is concluded that cyclin D1 overexpression alone does not induce a marked increase in the proliferative activity of ACC cells and that expression of this protein is not linked to poor prognosis in adenoid cystic carcinoma of the salivary gland.
腺样囊性癌是一种起源于唾液腺的恶性肿瘤。它往往生长缓慢，但易频繁复发和转移。细胞周期蛋白D1是一种细胞周期调节蛋白，据报道在各种类型的癌症中均有过表达，且与患者的不良预后相关。然而，细胞周期蛋白D1在唾液腺腺样囊性癌中的预后意义尚未确定。为了评估细胞周期蛋白D1在腺样囊性癌生物学调节中的作用，我们在一个内源性细胞周期蛋白D1高表达的既定腺样囊性癌细胞系中组成性表达反义细胞周期蛋白D1互补DNA（cDNA）。检测了细胞周期蛋白D1表达对体外细胞生长和细胞周期的影响。此外，我们还检测了31例唾液腺腺样囊性癌中细胞周期蛋白D1蛋白的免疫组化表达，并将其表达与增殖活性或预后相关联。反义细胞周期蛋白D1克隆与对照克隆在体外生长以及G1期和S期总细胞群体百分比方面没有显著差异。在接受检查的手术标本来源的肿瘤中，32%的肿瘤细胞周期蛋白D1蛋白免疫组化呈阳性。未发现细胞周期蛋白D1表达与细胞增殖或患者临床结局之间存在关联。结论是，单纯细胞周期蛋白D1过表达不会导致腺样囊性癌细胞增殖活性显著增加，且该蛋白的表达与唾液腺腺样囊性癌的不良预后无关。
