[通过酞菁敏化光氧化反应交联牛心包生物人工心脏瓣膜]

[Crosslinking bovine pericardial bioprosthetic heart valves by phthalocyanine sensitized photooxidation reaction].

作者信息

Lin Lin, Tang Ying-wu, Liang Qiang, Zhou Jian-ye, Cui Jing-wei, Hu Sheng-shou, Zhang Fu-shi

机构信息

Department of Chemistry, Tsinghua University, Beijing 100084, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Dec;25(6):671-5.

PMID:14714309

Abstract

OBJECTIVE

To crosslink bovine pericardial valves by phthalocyanine sensitized photooxidation reaction.

METHODS

Fresh bovine pericardial tissues were immersed in PBS solution containing 0.01% cupreous sulfonatophthalocyanine and exposed to a broad wavelength light source for 20-120 h. Using sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) to analyze the cross-linking extent of pericardial tissue. With the help of attenuated total reflection-fourier transform infrared (ATR-FTIR), explained the change of protein structure after photooxidation reaction. Differential scanning calorimetry (DSC) was used to measure the change of shrinkage temperature. Tissue water content experiment method was also used.

RESULTS

Photooxidized tissue was resistant to extraction when it was compared with untreated tissue and had similar crosslinking extent over glutaraldehyde-treated tissue. The three dimension structure of collagen were changed but not seriously destroyed and the model of photooxidation reaction was validated. Phthalocyanine-treated tissue was much more flexible than glutaraldehyde-treated tissue.

CONCLUSION

Bovine pericardial tissue can be successfully crosslinked by phthalocyanine-mediated photooxidation reaction.

摘要

目的

通过酞菁敏化光氧化反应交联牛心包瓣膜。

方法

将新鲜牛心包组织浸入含0.01%磺化铜酞菁的磷酸盐缓冲液（PBS）中，并用宽波长光源照射20 - 120小时。采用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析心包组织的交联程度。借助衰减全反射 - 傅里叶变换红外光谱（ATR - FTIR）解释光氧化反应后蛋白质结构的变化。使用差示扫描量热法（DSC）测量收缩温度的变化。还采用了组织含水量实验方法。