用于检测黄病毒的分子扩增检测法。

Molecular amplification assays for the detection of flaviviruses.

作者信息

Lanciotti Robert S

机构信息

Diagnostic and Reference Laboratory, Arbovirus Diseases Branch, Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA.

出版信息

Adv Virus Res. 2003;61:67-99. doi: 10.1016/s0065-3527(03)61002-x.

DOI:10.1016/s0065-3527(03)61002-x

PMID:14714430

Abstract

Over the past 10 years, a number of molecular amplification assays have been developed for the detection of flaviviruses. Most of these assays utilize the reverse transcriptase-polymerase chain reaction (RT-PCR) as the amplification format with detection by either agarose gel electrophoresis and ethidium bromide staining or hybridization with molecular probes. Recently, a modification of the standard RT-PCR using fluorescent-labeled oligonucleotide probes for detection (TaqMan) has been described. As a result, several assays for detecting flaviviruses have been developed using this approach. In addition, another amplification format, nucleic acid sequence based amplification (NASBA), has been developed and utilized for the detection of several flaviviruses. The various assay formats will be described and their utility discussed.

摘要

在过去10年里，已经开发出多种用于检测黄病毒的分子扩增检测方法。这些检测方法大多采用逆转录聚合酶链反应（RT-PCR）作为扩增形式，通过琼脂糖凝胶电泳和溴化乙锭染色或与分子探针杂交进行检测。最近，有人描述了一种对标准RT-PCR的改进方法，即使用荧光标记的寡核苷酸探针进行检测（TaqMan）。因此，已经开发出了几种使用这种方法检测黄病毒的检测方法。此外，另一种扩增形式，即基于核酸序列的扩增（NASBA），也已被开发出来并用于检测多种黄病毒。将对各种检测形式进行描述并讨论其效用。