一种用于检测蚊媒黄病毒的单管逆转录聚合酶链反应检测法。
A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses.
作者信息
Ayers M, Adachi D, Johnson G, Andonova M, Drebot M, Tellier R
机构信息
Metabolism Research Program, Hospital for Sick Children, Toronto, Ont., Canada.
出版信息
J Virol Methods. 2006 Aug;135(2):235-9. doi: 10.1016/j.jviromet.2006.03.009. Epub 2006 May 2.
Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, 3 and 4, West Nile virus, Kunjin virus (a clade of West Nile virus), and St. Louis encephalitis virus.
蚊媒黄病毒包括几种重要的人类疾病病原体，并为新发感染提供了显著例证。在本研究中，我们展示了一种单管逆转录聚合酶链反应（RT-PCR）检测方法的设计与验证，该方法使用一对共有引物来检测蚊媒黄病毒。扩增子测序可实现病毒种类鉴定。使用黄热病病毒疫苗株以及登革病毒1型、2型、3型和4型、西尼罗河病毒、库京病毒（西尼罗河病毒的一个分支）和圣路易斯脑炎病毒的代表性毒株的RNA对该检测方法进行了验证。
Zotero 插件