Polevoĭ V V, Tarakhovskaia E R, Maslov Iu I, Polevoĭ A V
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034 Russia.
Ontogenez. 2003 Nov-Dec;34(6):432-7.
We studied the effects of auxin (indolyl-3 acetic acid) on formation of the primary polarity axis in zygotes of the brown algae Fucus vesiculosus. Within the first 2.5 h after fertilization, the zygotes release this phytohormone in the environment. The treatment of developing zygotes with the inhibitor of indolyl-3-acetic acid transport from the cell triiodobenzoic acid at 5 mg/l arrests the auxin secretion and leads to its accumulation in the cells. This causes a significant delay in zygote polarization. The treatment of zygotes with the exogenous indolyl-3-acetic acid at 1 mg/l stimulates cell polarization and formation of a rhizoid process. When auxin was added to the medium with triiodobenzoic acid, the inhibitory effect of the latter was fully relieved. It has been proposed that the content of indolyl-3-acetic acid in the environment is a key factor in the induction of polarity of the F. vesiculosus zygotes.
我们研究了生长素（吲哚 - 3 - 乙酸）对褐藻墨角藻合子中初级极性轴形成的影响。受精后的最初2.5小时内，合子会将这种植物激素释放到环境中。用5毫克/升的吲哚 - 3 - 乙酸从细胞中运输的抑制剂三碘苯甲酸处理发育中的合子，会抑制生长素分泌并导致其在细胞中积累。这会导致合子极化显著延迟。用1毫克/升的外源吲哚 - 3 - 乙酸处理合子会刺激细胞极化并形成假根突起。当将生长素添加到含有三碘苯甲酸的培养基中时，后者的抑制作用会完全解除。有人提出，环境中吲哚 - 3 - 乙酸的含量是诱导墨角藻合子极性的关键因素。
