He Yonghong, Wang Ruikang K
Institute of Bioscience and Technology, Cranfield Biomedical Centre, Cranfield University at Silsoe, Bedfordshire MK45 4DT, United Kingdom.
J Biomed Opt. 2004 Jan-Feb;9(1):200-6. doi: 10.1117/1.1629682.
The depth of light penetration into highly scattering tissues can be improved by the application of biocompatible and osmotically active chemical agents. We compare the dynamics of optical clearing of tissue by the topical application of glycerol and dimethyl sulfoxide (DMSO) using optical coherence tomography (OCT). It is demonstrated experimentally that both agents can largely improve the OCT imaging depth for porcine stomach tissue. During a period of approximately 20 to 30 min after the application of glycerol image contrast is also enhanced. This enhancement disappears over time. Such enhancement of image contrast is not observed with DMSO. Glycerol causes a higher degree of dehydration of the tissue than DMSO does. We suggest that these phenomena are caused by a two-stage diffusion of the chemicals. The first stage of diffusion is from the top tissue to the intercellular space, and the second is into the cell matrix. During the first stage, the imaging contrast could be improved by dehydration.
通过应用生物相容性和渗透活性化学试剂，可以提高光穿透高散射组织的深度。我们使用光学相干断层扫描（OCT）比较了局部应用甘油和二甲基亚砜（DMSO）时组织光学清除的动力学。实验表明，这两种试剂都能在很大程度上提高猪胃组织的OCT成像深度。在应用甘油后的大约20到30分钟内，图像对比度也会增强。这种增强会随着时间消失。使用DMSO时未观察到这种图像对比度的增强。甘油导致的组织脱水程度比DMSO更高。我们认为这些现象是由化学物质的两阶段扩散引起的。扩散的第一阶段是从组织表层到细胞间隙，第二阶段是进入细胞基质。在第一阶段，成像对比度可通过脱水得到改善。
