Yu Hyeonseung, Lee Peter, Jo YoungJu, Lee KyeoReh, Tuchin Valery V, Jeong Yong, Park YongKeun
Korea Advanced Institute of Science and Technology, Department of Physics, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreabKAIST Institute of Health Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
KAIST Institute of Health Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of KoreacKorea Advanced Institute of Science and Technology, Department of Bio and Brain Engineering, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
J Biomed Opt. 2016 Dec 1;21(12):121510. doi: 10.1117/1.JBO.21.12.121510.
We demonstrate that simultaneous application of optical clearing agents (OCAs) and complex wavefront shaping in optical coherence tomography (OCT) can provide significant enhancement of penetration depth and imaging quality. OCA reduces optical inhomogeneity of a highly scattering sample, and the wavefront shaping of illumination light controls multiple scattering, resulting in an enhancement of the penetration depth and signal-to-noise ratio. A tissue phantom study shows that concurrent applications of OCA and wavefront shaping successfully operate in OCT imaging. The penetration depth enhancement is further demonstrated for <italic<ex vivo</italic< mouse ears, revealing hidden structures inaccessible with conventional OCT imaging.
我们证明，在光学相干断层扫描（OCT）中同时应用光学透明剂（OCA）和复杂波前整形可以显著提高穿透深度和成像质量。OCA降低了高散射样品的光学不均匀性，照明光的波前整形控制了多重散射，从而提高了穿透深度和信噪比。一项组织模型研究表明，OCA和波前整形的同时应用在OCT成像中成功发挥作用。对于离体小鼠耳朵，进一步证明了穿透深度的增加，揭示了传统OCT成像无法触及的隐藏结构。
