Bagley Kenneth C, Abdelwahab Sayed F, Tuskan Robert G, Lewis George K
Division of Vaccine Research, Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland School of Medicine, University of Maryland Baltimore, Baltimore, Maryland 21201, USA.
Clin Diagn Lab Immunol. 2004 Jan;11(1):77-82. doi: 10.1128/cdli.11.1.77-82.2004.
Calcium is an important second messenger in the phospholipase C (PLC) signal transduction pathway. Calcium signaling is involved in many biological processes, including muscle contraction, cellular activation, and cellular proliferation. Dendritic cell (DC) maturation is induced by many different stimuli, including bacterial lipopolysaccharide (LPS), bacterial toxins, inflammatory cytokines, prostaglandins, as well as calcium mobilization. In the present study, we determined the role of the PLC signal transduction pathway in the activation and maturation of human monocyte-derived DCs (MDDCs) induced by diverse agonists. We found that signaling through PLC activates MDDCs to mature and is necessary for LPS, cholera toxin, dibutyryl-cyclic AMP, prostaglandin E2, and the calcium ionophore A23187 to induce MDDC maturation. The results of the present study along with the results of other studies indicate that multiple signaling pathways are involved in the activation of DCs and that inhibition of any of these pathways inhibits the maturation of DCs.
钙是磷脂酶C（PLC）信号转导途径中一种重要的第二信使。钙信号传导参与许多生物学过程，包括肌肉收缩、细胞活化和细胞增殖。树突状细胞（DC）成熟可由多种不同刺激诱导，包括细菌脂多糖（LPS）、细菌毒素、炎性细胞因子、前列腺素以及钙动员。在本研究中，我们确定了PLC信号转导途径在多种激动剂诱导的人单核细胞衍生树突状细胞（MDDC）活化和成熟中的作用。我们发现，通过PLC的信号传导可激活MDDC使其成熟，并且对于LPS、霍乱毒素、二丁酰环磷酸腺苷、前列腺素E2以及钙离子载体A23187诱导MDDC成熟而言是必需的。本研究结果以及其他研究结果表明，多种信号转导途径参与DC的活化，并且抑制这些途径中的任何一种都会抑制DC的成熟。
