Chng Wee J, Tan Guat B, Kuperan Ponnudurai
Department of Haematology-Oncology, National University Hospital, Singapore, Republic of Singapore.
Clin Diagn Lab Immunol. 2004 Jan;11(1):168-73. doi: 10.1128/cdli.11.1.168-173.2004.
We established a normal reference range for peripheral blood lymphocyte subsets in a multiracial adult population by using single-platform flow cytometry. Further analysis of our cohort showed that the CD8+-cell counts decrease with age, there is a gender difference in NK cell percentages and counts, and there are significant differences in the CD3+-, CD4+-, and CD19+-cell counts between Indians and other racial groups. Overall, our results are significantly different from other published data. This difference further stresses the need for different populations to establish their own reference ranges as these may have important implications for the management of patients with human immunodeficiency virus and AIDS. The use of single-platform flow cytometry will eliminate some of the variability between different study centers, making studies more comparable. This platform should be used for future studies into the effects of age, sex, and race on lymphocyte subsets.
我们通过单平台流式细胞术建立了多民族成年人群外周血淋巴细胞亚群的正常参考范围。对我们队列的进一步分析表明，CD8+细胞计数随年龄增长而下降，NK细胞百分比和计数存在性别差异，印度人与其他种族群体之间的CD3+、CD4+和CD19+细胞计数存在显著差异。总体而言，我们的结果与其他已发表的数据有显著差异。这种差异进一步强调了不同人群需要建立自己的参考范围，因为这些范围可能对人类免疫缺陷病毒和艾滋病患者的管理具有重要意义。使用单平台流式细胞术将消除不同研究中心之间的一些变异性，使研究更具可比性。该平台应用于未来关于年龄、性别和种族对淋巴细胞亚群影响的研究。
