Chang Syuan-Ting, Chuang Yi-Fang, Li Ai-Hsien, Fan Yang-Teng, Liao Man-Ru, Chen I-Yu, Hung Ruo-Wei, Yang Tienyu Owen, Chiu Yen-Ling
School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Institute of Public Health, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Immun Ageing. 2024 Oct 29;21(1):75. doi: 10.1186/s12979-024-00480-x.
The circulatory peripheral immune system is the most convenient approach for determining an individual's immune status. Due to various reasons, while previous studies have addressed the critical impact of age, most individual studies did not analyze immunosenescence in a systemic manner, which complicates the possibility of building a reference range for age-dependent immune profiles for effective immune monitoring. To address this gap, this study analyzed a group of healthy individuals to establish age-specific reference ranges of the healthy circulatory immune profile, and a systematic review and meta-analysis were conducted to validate the findings and create generalizable immune cell reference ranges.
Our study recruited a total of 363 healthy Taiwanese adults (median age 42 years [IQR 30, 62], age range 21 to 87 years, 43.3% male), including 158 under 40 years old, 127 between 40-64 years old, and 78 over 64 years old. Significant age-related alterations were observed in both adaptive and innate immune cell subsets. CD8 + T cells decreased and CD4/CD8 ratio increased, with notable increases in NK cells. CD4 + T cells were less impacted by aging, while CD8 + T cells significantly lost CD28 and increased CD31 expression with age. A clear reverse trend in naïve and memory subsets of CD4 + and CD8 + T cells was observed. Detailed reference ranges for immune cell subsets in healthy Taiwanese adults were established. A systematic review included 7,425 adults and a meta-analysis of 12 eligible studies confirmed our findings in Taiwan, enhancing generalizability.
Combined with previous studies and original data through a systematic review and meta-analysis, we highlighted and quantified significant immune profile differences between older and younger individuals. The sex and age-specific reference ranges for peripheral immune cell subsets can serve as a basis for effective immune monitoring of various aging-related illnesses.
循环外周免疫系统是确定个体免疫状态最便捷的途径。由于各种原因，尽管先前的研究已经探讨了年龄的关键影响，但大多数个体研究并未以系统的方式分析免疫衰老，这使得建立年龄依赖性免疫谱参考范围以进行有效免疫监测变得复杂。为了填补这一空白，本研究分析了一组健康个体，以建立健康循环免疫谱的年龄特异性参考范围，并进行了系统评价和荟萃分析，以验证研究结果并创建可推广的免疫细胞参考范围。
我们的研究共招募了363名健康的台湾成年人（年龄中位数42岁[四分位间距30, 62]，年龄范围21至87岁，男性占43.3%），其中158名年龄在40岁以下，127名年龄在40 - 64岁之间，78名年龄在64岁以上。在适应性和先天性免疫细胞亚群中均观察到了与年龄相关的显著变化。CD8+T细胞减少，CD4/CD8比值增加，自然杀伤细胞显著增加。CD4+T细胞受衰老影响较小，而CD8+T细胞随着年龄增长显著丧失CD28并增加CD31表达。在CD4+和CD8+T细胞的幼稚和记忆亚群中观察到明显的反向趋势。建立了健康台湾成年人免疫细胞亚群的详细参考范围。一项系统评价纳入了7425名成年人，对12项符合条件的研究进行的荟萃分析证实了我们在台湾的研究结果，增强了可推广性。
通过系统评价和荟萃分析结合先前的研究和原始数据，我们突出并量化了老年和年轻个体之间显著的免疫谱差异。外周免疫细胞亚群的性别和年龄特异性参考范围可为各种衰老相关疾病的有效免疫监测提供依据。
