Woywodt Alexander, Scheer Johanna, Hambach Lothar, Buchholz Stefanie, Ganser Arnold, Haller Hermann, Hertenstein Bernd, Haubitz Marion
Division of Nephrology, Department of Internal Medicine, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
Blood. 2004 May 1;103(9):3603-5. doi: 10.1182/blood-2003-10-3479. Epub 2004 Jan 8.
Damage to endothelial cells is the common feature of vascular disorders associated with hematopoietic stem cell transplantation (HSCT). Elevated numbers of circulating endothelial cells reflect the extent of endothelial damage in a variety of disorders but their use in HSCT has not been investigated so far. We studied 39 patients undergoing allogeneic HSCT with different conditioning regimens and 22 healthy controls. Circulating endothelial cells were enumerated with immunomagnetic isolation during the course of HSCT. After conditioning, cell numbers were significantly elevated (median 44 cells/mL) compared with baseline (median 16 cells/mL) and controls (median 8 cells/mL). Patients who received radiation had an earlier peak when compared with patients who received chemotherapy. Patients who received reduced-intensity conditioning had significantly lower cell numbers (median 24 cells/mL) than those who received standard conditioning. These observations provide a novel marker to investigate microvascular endothelial damage and the effects of different conditioning regimens in patients undergoing HSCT.
内皮细胞损伤是与造血干细胞移植（HSCT）相关的血管疾病的共同特征。循环内皮细胞数量的增加反映了多种疾病中内皮损伤的程度，但迄今为止尚未对其在HSCT中的应用进行研究。我们研究了39例接受不同预处理方案的异基因HSCT患者和22名健康对照者。在HSCT过程中，通过免疫磁珠分离法对循环内皮细胞进行计数。预处理后，细胞数量与基线（中位数16个细胞/毫升）和对照者（中位数8个细胞/毫升）相比显著升高（中位数44个细胞/毫升）。与接受化疗的患者相比，接受放疗的患者峰值出现得更早。接受减低强度预处理的患者细胞数量（中位数24个细胞/毫升）明显低于接受标准预处理的患者。这些观察结果为研究接受HSCT患者的微血管内皮损伤以及不同预处理方案的效果提供了一个新的标志物。
