Umezawa Eufrosina S, Luquetti Alejandro O, Levitus Gabriela, Ponce Carlos, Ponce Elisa, Henriquez Diana, Revollo Susana, Espinoza Bertha, Sousa Octavio, Khan Baldip, da Silveira José Franco
Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, UNIFESP, São Paulo, Brazil.
J Clin Microbiol. 2004 Jan;42(1):449-52. doi: 10.1128/JCM.42.1.449-452.2004.
An enzyme-linked immunosorbent assay to diagnose Chagas' disease by a serological test was performed with Trypanosoma cruzi recombinant antigens (JL8, MAP, and TcPo). High sensitivity (99.4%) and specificity (99.3%) were obtained when JL8 was combined with MAP (JM) and tested with 150 serum samples from chagasic and 142 nonchagasic individuals. Moreover, JM also diagnosed 84.2% of patients in the acute phase of T. cruzi infection.
采用克氏锥虫重组抗原（JL8、MAP和TcPo）通过血清学检测进行酶联免疫吸附测定以诊断恰加斯病。当JL8与MAP（JM）联合使用并对150份恰加斯病患者血清样本和142份非恰加斯病患者血清样本进行检测时，获得了高灵敏度（99.4%）和高特异性（99.3%）。此外，JM还诊断出84.2%处于克氏锥虫感染急性期的患者。
