Wang Jian, Li Fang, Zhang Wei, Wang Jie, Gao Haiyan, Wei Suping, Zhang Shibi, Ran Junguo, Gou Li, Zhou Song
Sichuan Cancer Hosiptal, Chengdu 610041.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Dec;20(4):615-7.
In this study we deliberated over the principles and methods and then took the noninvasive continuous measurement of blood glucose concentration through the skin of rabbits. The glucose oxidase sensor was made by covalent immobilization. The best making method of sensor and stable working condition were sifted. Ten female and 10 male adult white rabbits were allocated into the groups of the ante-ultrasound and post-ultrasound, the injection of glucose, and the high and low frequency ultrasounds. After the skin surface was treated by high or low frenquency ultrasound for 5 minutes on the rabbits, obvious changes (P < 0.01) of post-ultrasound and post-injection of glucose were observed by means of glucose oxidase sensor and microcurrent apparatus. After application of ultrasound to the skin of rabbits, the penetration of glucose through the rabbit skin increased obviously. The change of microcurrent signal that was exchanged by the glucose sensor correlated positively with the concentration of glucose of rabbit body. The blood glucose can be tested by the glucose sensor on the skin surface of living animal.
在本研究中，我们商讨了原理和方法，然后对家兔皮肤进行无创连续血糖浓度测量。葡萄糖氧化酶传感器通过共价固定法制备。筛选出了传感器的最佳制作方法和稳定的工作条件。将10只成年雌性和10只成年雄性白兔分为超声前、超声后、注射葡萄糖以及高频和低频超声组。对家兔皮肤表面进行5分钟的高频或低频超声处理后，通过葡萄糖氧化酶传感器和微电流仪观察到超声后及注射葡萄糖后有明显变化（P<0.01）。对家兔皮肤施加超声后，葡萄糖透过家兔皮肤的渗透率明显增加。葡萄糖传感器所交换的微电流信号变化与兔体血糖浓度呈正相关。血糖可通过活体动物皮肤表面的葡萄糖传感器进行检测。
