Saeger S, Preidel W, von Lucadou I, Ruprecht L, Lager W
Physiology and Biocybernetics Institute, University of Erlangen, FRG.
Biomed Biochim Acta. 1991;50(7):885-91.
In an animal experiment with the electrocatalytic glucose sensor, measurements were carried out over one week in the extracorporeal circulation of a sheep. Glucose tolerance tests were performed, and the influence of increased urea concentrations in the blood on the glucose determination was investigated. The sensor constructed as a flow-through cell was integrated via a vascular graft outside the body into the carotid artery of the animal and activated by an external electronic unit of measurement. The glucose concentration was determined by measuring the impedance of the electrode/membrane system at various potentials. By means of a subsequent correlation analysis of the measured values obtained over one week, a calibration valid for the entire measurement period was established. After a zero adjustment, it was even possible to adopt the calibration from the glucose measurement of the preceding animal experiment. The investigations of the influence of urea on the glucose measurement showed that the error in measurement of the sensor, which is 20% on average, is only insignificantly increased when the urea level is raised beyond the maximum physiological concentration.
在一项使用电催化葡萄糖传感器的动物实验中，在绵羊的体外循环中进行了为期一周的测量。进行了葡萄糖耐量试验，并研究了血液中尿素浓度升高对葡萄糖测定的影响。构建为流通池的传感器通过血管移植物在体外整合到动物的颈动脉中，并由外部电子测量单元激活。通过在不同电位下测量电极/膜系统的阻抗来测定葡萄糖浓度。通过对一周内获得的测量值进行后续的相关性分析，建立了适用于整个测量期的校准。经过零点调整后，甚至可以采用前一个动物实验中葡萄糖测量的校准。尿素对葡萄糖测量影响的研究表明，传感器的测量误差平均为20%，当尿素水平升高超过最大生理浓度时，误差仅略有增加。
