Sousa-Majer Maria José de, Turner Neil C, Hardie Darryl C, Morton Roger L, Lamont Byron, Higgins Thomas J V
Department of Environmental Biology, Curtin University of Technology PO Box U1987, Perth, WA 6845, Australia.
J Exp Bot. 2004 Feb;55(396):497-505. doi: 10.1093/jxb/erh037. Epub 2004 Jan 12.
The effects of water deficit and high temperature on the production of alpha-amylase inhibitor 1 (alpha-AI-1) were studied in transgenic peas (Pisum sativum L.) that were developed to control the seed-feeding pea weevil (Bruchus pisorum L., Coleoptera: Bruchidae). Transgenic and non-transgenic plants were subjected to water-deficit and high-temperature treatments under controlled conditions in the glasshouse and growth cabinet, beginning 1 week after the first pods were formed. In the water-deficit treatments, the peas were either adequately watered (control) or water was withheld after first pod formation. The high-temperature experiments were performed in two growth cabinets, one maintained at 27/22 degrees C (control) and one at 32/27 degrees C day/night temperatures, with the vapour pressure deficit maintained at 1.3 kPa. The plants exposure to high temperatures and water deficit produced 27% and 79% fewer seeds, respectively, than the controls. In the transgenic peas the level of alpha-AI-1 as a percentage of total protein was not influenced by water stress, but was reduced on average by 36.3% (the range in two experiments was 11-50%) in the high-temperature treatment. Transgenic and non-transgenic pods of plants grown at 27/22 degrees C and 32/27 degrees C were inoculated with pea weevil eggs to evaluate whether the reduction in level of alpha-AI-1 in the transgenic pea seeds affected pea weevil development and survival. At the higher temperatures, 39% of adult pea weevil emerged, compared to 1.2% in the transgenic peas grown at the lower temperatures, indicating that high temperature reduced the protective capacity of the transgenic peas.
在为控制以种子为食的豌豆象鼻虫（豆象科豌豆象，鞘翅目：豆象科）而培育的转基因豌豆（豌豆）中，研究了水分亏缺和高温对α-淀粉酶抑制剂1（α-AI-1）产生的影响。在温室和生长箱的可控条件下，从第一批豆荚形成后1周开始，对转基因和非转基因植株进行水分亏缺和高温处理。在水分亏缺处理中，豌豆要么得到充足浇水（对照），要么在第一批豆荚形成后停止浇水。高温实验在两个生长箱中进行，一个保持在27/22摄氏度（对照），另一个保持在日/夜温度为32/27摄氏度，水汽压差保持在1.3千帕。与对照相比，遭受高温和水分亏缺的植株分别少结27%和79%的种子。在转基因豌豆中，α-AI-1占总蛋白的百分比水平不受水分胁迫影响，但在高温处理中平均降低了36.3%（两个实验中的范围为11 - 50%）。对在27/22摄氏度和32/27摄氏度下生长的植株的转基因和非转基因豆荚接种豌豆象鼻虫卵，以评估转基因豌豆种子中α-AI-1水平的降低是否影响豌豆象鼻虫的发育和存活。在较高温度下，39%的成年豌豆象鼻虫羽化，而在较低温度下生长的转基因豌豆中这一比例为1.2%，这表明高温降低了转基因豌豆的保护能力。
