de Sousa-Majer Maria José, Hardie Darryl C, Turner Neil C, Higgins Thomas J V
Department of Environmental Biology, Curtin University of Technology, P.O. Box U1987, Perth, WA 6845, Australia.
J Econ Entomol. 2007 Aug;100(4):1416-22. doi: 10.1603/0022-0493(2007)100[1416:baiitp]2.0.co;2.
This glasshouse study used an improved larval measurement procedure to evaluate the impact of transgenic pea, Pisum sativum L., seeds expressing a-amylase inhibitor (AI)-1 or -2 proteins on pea weevil, Bruchus pisorum L. Seeds of transgenic 'Laura' and 'Greenfeast' peas expressing alpha-(AI)-1 reduced pea weevil survival by 93-98%. Larval mortality occurred at an early instar. Conversely, in nontransgenic cultivars, approximately 98-99% of the pea weevils emerged as adults. By measuring the head capsule size, we determined that larvae died at the first to early third instar in alpha-(AI)-1 transgenic peas, indicating that this inhibitor is highly effective in controlling this insect. By contrast, transgenic Laura and 'Dundale' expressing alpha-(AI)-2 did not affect pea weevil survival, but they did delay larval development. After 77 d of development, the head capsule size indicated that the larvae were still at the third instar stage in transgenic alpha-(AI)-2 peas, whereas adult bruchids had developed in the nontransgenic peas.
这项温室研究采用了一种改进的幼虫测量程序，以评估表达α-淀粉酶抑制剂（AI）-1或-2蛋白的转基因豌豆（Pisum sativum L.）种子对豌豆象（Bruchus pisorum L.）的影响。表达α-（AI）-1的转基因“劳拉”和“绿 feast”豌豆种子使豌豆象的存活率降低了93%至98%。幼虫死亡率发生在早期龄期。相反，在非转基因品种中，约98%至99%的豌豆象发育为成虫。通过测量头壳大小，我们确定在α-（AI）-1转基因豌豆中，幼虫在第一龄至第三龄早期死亡，这表明这种抑制剂在控制这种昆虫方面非常有效。相比之下，表达α-（AI）-2的转基因“劳拉”和“邓代尔”对豌豆象的存活率没有影响，但确实延迟了幼虫发育。经过77天的发育，头壳大小表明转基因α-（AI）-2豌豆中的幼虫仍处于第三龄期，而在非转基因豌豆中豌豆象成虫已经发育。
