Maila Hazel T, Bowyer Sheila M, Swanepoel Robert
National Institute for Communicable Diseases and Department of Medical Virology, University of Witwatersrand, Private Bag X4, Sandringham 2131, South Africa.
J Gen Virol. 2004 Jan;85(Pt 1):89-95. doi: 10.1099/vir.0.19587-0.
Endemic circulation of hepatitis E virus (HEV) in Namibia was suspected from serological data during an outbreak of non-A, non-B hepatitis in Rundu in 1995. The source of the outbreak was suspected to be the water supply, which had been compromised approximately 6 months earlier. Four HEV isolates from four different persons in this outbreak were successfully amplified, sequenced and analysed over a 451 bp region of a subgenomic fragment from the 3' end of the genome in ORF2. Phylogenetic analysis showed that the four Namibian HEV isolates clustered with a Mexican isolate in genotype II and shared 85.8-86.3 % nucleotide identity with the 1987 Mexican isolate, but were only 77.6-79.6 % similar to other African isolates. HEV isolated from the same region of Namibia in 1983 was reported to cluster in genotype I. However, virus isolates from sporadic cases of HEV isolated in 1997/8 in Nigeria were also from genotype II.
1995年在伦杜发生非甲非乙型肝炎疫情期间，根据血清学数据怀疑纳米比亚存在戊型肝炎病毒（HEV）的地方性流行。疫情源头怀疑是供水系统，大约在6个月前该系统受到了影响。在此次疫情中，从4名不同患者身上成功扩增、测序并分析了4株HEV分离株，分析区域为开放阅读框2（ORF2）中基因组3'端亚基因组片段的451 bp区域。系统发育分析表明，这4株纳米比亚HEV分离株与1株墨西哥分离株聚集在基因型II中，与1987年墨西哥分离株的核苷酸同一性为85.8 - 86.3%，但与其他非洲分离株的相似性仅为77.6 - 79.6%。据报道，1983年从纳米比亚同一地区分离出的HEV聚集在基因型I中。然而，1997/1998年在尼日利亚散发的HEV病例中分离出的病毒株也属于基因型II。
