Li Lingjun, Zhu Yonghong, Fu Hongwei, Wei Xianfei, Wang Ling, Liang Jingrui, Ji Yanli, Tang Ronglan, Zhuang Hui
Department of Microbiology, Peking University Health Science Center, Beijing, China.
Liver Int. 2009 Sep;29(8):1230-40. doi: 10.1111/j.1478-3231.2009.02012.x. Epub 2009 Apr 15.
Hepatitis E virus (HEV) is one of the leading causes of the enteric-transmitted acute hepatitis. Many studies have found high identities between human and animal HEV isolates using partial sequence comparison analysis.
To determine and phylogenetically analyse the complete genome of the swGX40 isolate from the Guangxi Zhuang autonomous region.
The overlapping fragments of HEV isolate swGX40 were amplified with reverse transcription-nested polymerase chain reaction (PCR) and the 5' and 3' ends of viral genome were amplified with rapid amplification of cDNA ends. The PCR products were cloned and sequenced. The sequence and phylogenetic analysis of swGX40 were performed.
The full genome of the swGX40 strain consisted of 7233 nucleotides, excluding the poly (A) tail of 36 residues. There are three open reading frames (ORFs), encoding 1705, 674 and 114 amino acids (aa) respectively. The full-genomic sequencing showed that the swGX40 strain shared similarity with all known HEV genotype 1, 2 and 3 isolates by 73.4-76.5% and with an identity of 83.1-91.2% among genotype 4 HEV isolates. The partial ORF2 sequencing (249 nt) showed that swGX40 shared a high nucleotide identity of 94 and 97% with the Chinese human strain LZ-105 and the Vietnamese human strain HE-JVN-1 respectively.
The swine isolate swGX40 was closely related to the human isolate LZ-105, both of which were collected from Liuzhou, the same district in the Guangxi Zhuang autonomous region. This molecular biological evidence strongly supported the zoonosis hypothesis of hepatitis E.
戊型肝炎病毒（HEV）是经肠道传播的急性肝炎的主要病因之一。许多研究通过部分序列比较分析发现人和动物的HEV分离株之间具有高度同源性。
测定并系统发育分析来自广西壮族自治区的swGX40分离株的全基因组。
采用逆转录巢式聚合酶链反应（PCR）扩增HEV分离株swGX40的重叠片段，用cDNA末端快速扩增法扩增病毒基因组的5′和3′末端。将PCR产物克隆并测序。对swGX40进行序列和系统发育分析。
swGX40株全基因组由7233个核苷酸组成，不包括36个残基的聚（A）尾。有三个开放阅读框（ORF），分别编码1705、674和114个氨基酸（aa）。全基因组测序显示，swGX40株与所有已知的1、2和3型HEV分离株的相似性为73.4% - 76.5%，与4型HEV分离株之间的同源性为83.1% - 91.2%。部分ORF2测序（249 nt）显示，swGX40与中国人类株LZ - 105和越南人类株HE - JVN - 1的核苷酸同源性分别高达94%和97%。
猪分离株swGX40与人类分离株LZ - 105密切相关，二者均采集自广西壮族自治区同一地区柳州市。这一分子生物学证据有力支持了戊型肝炎的人畜共患病假说。
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