Ono Etsuro, Amagai Keiko, Yoshino Saori, Taharaguchi Satoshi, Inobe Manabu, Uede Toshimitsu
Laboratory of Animal Experiment for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
Sankyo Labo Service Corporation, Tokyo 132-0023, Japan.
J Gen Virol. 2004 Jan;85(Pt 1):173-178. doi: 10.1099/vir.0.19481-0.
Porcine herpesvirus entry mediator C (HveC) is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine HveC mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) and bovine herpesvirus type 1 (BHV-1). In order to assess the antiviral potential of a soluble form of porcine HveC, Vero cells were transformed with the chimeric gene expressing a fusion protein (PHveCIg) consisting of an extracellular domain of porcine HveC and the Fc portion of human IgG1. The transformed cell lines expressing PHveCIg showed marked resistance to PRV infection. Resistance to infection by other alphaherpesviruses (HSV-1 and BHV-1) was also observed in the transformed cell line. The present results demonstrate that a soluble form of porcine HveC is able to exert a significant antiviral effect against pseudorabies virus and other alphaherpesvirus infection in vitro.
猪疱疹病毒进入介质C（HveC）是一种与病毒粒子糖蛋白D（gD）结合的α疱疹病毒受体。猪HveC介导伪狂犬病病毒（PRV）、1型和2型单纯疱疹病毒（HSV-1和HSV-2）以及1型牛疱疹病毒（BHV-1）的进入。为了评估可溶性形式的猪HveC的抗病毒潜力，用表达由猪HveC的细胞外结构域和人IgG1的Fc部分组成的融合蛋白（PHveCIg）的嵌合基因转化Vero细胞。表达PHveCIg的转化细胞系对PRV感染表现出明显的抗性。在转化细胞系中也观察到对其他α疱疹病毒（HSV-1和BHV-1）感染的抗性。目前的结果表明，可溶性形式的猪HveC能够在体外对伪狂犬病病毒和其他α疱疹病毒感染发挥显著的抗病毒作用。
