Ghadersohi Ali, Odunsi Kunle, Lele Shashikant, Collins Yvonne, Greco William R, Winston Janet, Liang Ping, Sood Ashwani K
Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Oncol Rep. 2004 Feb;11(2):453-8.
We previously reported that prostate derived Ets transcription factor (PDEF) is a breast tumor-associated molecule. To obtain further insights into PDEF expression in other human tumor types, a cDNA library database from human adult normal and tumor tissues was compiled and searched for PDEF distribution. The results showed that PDEF is present at relative higher frequencies in the cDNA libraries from brain, breast, lung and ovarian tumors in comparison to those from the corresponding normal tissues. RT/PCR analysis of PDEF expression in ovarian tumors confirmed that PDEF is expressed in 36 out of 51 (71%) ovarian tumors. Further comparison of the distribution of PDEF with other widely recognized cancer-associated molecules showed that PDEF has more restricted distributions than Her-2/neu, Bcl-2, survivin or telomerase in cDNA libraries from normal human tissues and more increased distribution than Her-2/neu, CA-125, Bcl-2, survivin and telomerase in cDNA libraries from brain (except survivin), breast, lung and ovarian tumors. These data together show a better tumor-association for PDEF and suggest that PDEF is a more suitable target for developing specific cancer therapies.
我们之前报道过，前列腺衍生的Ets转录因子（PDEF）是一种与乳腺肿瘤相关的分子。为了进一步深入了解PDEF在其他人类肿瘤类型中的表达情况，我们编制了一个来自人类成人正常组织和肿瘤组织的cDNA文库数据库，并搜索PDEF的分布情况。结果显示，与相应正常组织的cDNA文库相比，PDEF在脑、乳腺、肺和卵巢肿瘤的cDNA文库中出现的频率相对较高。对卵巢肿瘤中PDEF表达的RT/PCR分析证实，51例卵巢肿瘤中有36例（71%）表达PDEF。将PDEF的分布与其他广泛认可的癌症相关分子进行进一步比较，结果表明，在来自正常人类组织的cDNA文库中，PDEF的分布比Her-2/neu、Bcl-2、生存素或端粒酶更具局限性；而在来自脑（生存素除外）、乳腺、肺和卵巢肿瘤的cDNA文库中，PDEF的分布比Her-2/neu、CA-125、Bcl-2、生存素和端粒酶增加得更多。这些数据共同表明PDEF与肿瘤的关联性更强，并提示PDEF是开发特异性癌症治疗方法更合适的靶点。
