Sibila Marina, Calsamiglia Maria, Segalés Joaquim, Blanchard Philippe, Badiella Llorenç, Le Dimna Mireille, Jestin André, Domingo Mariano
Centre de Recerca en Sanitat Animal, Departament de Sanitat i d'Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Am J Vet Res. 2004 Jan;65(1):88-92. doi: 10.2460/ajvr.2004.65.88.
To determine whether correlations exist between viremia with porcine circovirus type 2 (PCV2) and serum antibody profiles and between detection of PCV2 in nasal cavities and viremia of pigs from farms with and without postweaning multisystemic wasting syndrome (PMWS).
495 pigs, ranging from the late nursery stage to the early grower-finisher stage of production.
Serum antibodies to PCV2 were studied with an ELISA that detects the ORF2 viral protein. Nasal swab specimens and serum samples were tested with a PCV2-specific PCR assay.
PCV2 DNA and serum antibodies to PCV2 were detected in pigs from all farms, although in different proportions. Overall, PCV2 DNA was detected in greater percentages in serum samples and nasal swab specimens of pigs from farms with PMWS. Although viral DNA was detected in both serum samples and nasal swab specimens, PCV2 detection in nasal swab specimens was higher than in serum samples of pigs from all farms. Serum antibodies to PCV2 were detected in a greater percentage of pigs from farms with PMWS, compared with farms without PMWS.
A high prevalence of PCV2 infection was found in pigs from farms with and without PMWS. Besides the presence of PCV2, unknown additional factors may be necessary to induce the full expression of PMWS.
确定2型猪圆环病毒（PCV2）病毒血症与血清抗体谱之间，以及在有和没有断奶后多系统消耗综合征（PMWS）的猪场中，猪鼻腔内PCV2检测与病毒血症之间是否存在相关性。
495头猪，处于生产的保育后期至生长育肥前期阶段。
采用检测ORF2病毒蛋白的ELISA法研究PCV2血清抗体。用PCV2特异性PCR检测法检测鼻拭子标本和血清样本。
所有猪场的猪均检测到PCV2 DNA和PCV2血清抗体，不过比例不同。总体而言，在患有PMWS的猪场的猪的血清样本和鼻拭子标本中，检测到PCV2 DNA的百分比更高。虽然在血清样本和鼻拭子标本中均检测到病毒DNA，但所有猪场的猪鼻拭子标本中的PCV2检测率高于血清样本。与没有PMWS的猪场相比，患有PMWS的猪场中检测到PCV2血清抗体的猪的比例更高。
在有和没有PMWS的猪场的猪中均发现PCV2感染的高流行率。除了存在PCV2外，可能还需要未知的其他因素来诱导PMWS的充分表现。
