O'Connor Kim C, Song Hong, Rosenzweig Nitsa, Jansen David A
Department of Chemical Engineering, Tulane University, Boggs Center, Room 300, New Orleans, LA 70118, USA.
Biotechnol Lett. 2003 Dec;25(23):1967-72. doi: 10.1023/b:bile.0000004386.08923.ab.
The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% +/- 7% on Matrigel. Lipid volume surpassed 4.5 x 10(3) microm3 cell(-1) for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3 x 10(3) microm2 cell(-1). These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.
将人脂肪组织的基质血管部分置于不同细胞外基质底物上进行体外脂肪生成。脂肪组织取自25至45岁接受择期手术的女性患者的乳房。24天后，在纤连蛋白上，不到5%的基质血管细胞转化为脂肪细胞；在组织培养塑料和I型胶原上，这一比例为13%至28%；在基质胶上则为59%±7%。基质胶上的脂质体积超过4.5×10³立方微米/细胞，其他底物上的脂质体积则低30%。基质胶和纤连蛋白上细胞增殖明显，纤连蛋白上细胞铺展最为显著，投影面积超过3×10³平方微米/细胞。这些结果与脂肪植入物的设计相关，有助于深入了解其可行性和支架组成。
