Rowiński Paweł, Bilewicz Renata, Stébé Marie-José, Rogalska Ewa
Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland, and Equipe Physico-chimie des Colloïdes, UMR 7565 CNRS, Faculté des Sciences, Université Henri Poincaré Nancy 1, BP 239, 54506 Vandoeuvre-lès-Nancy, Cedex, France.
Anal Chem. 2004 Jan 15;76(2):283-91. doi: 10.1021/ac034612o.
An enzyme-catalyzed process has been used for dioxygen monitoring. The enzymes were two different laccases (p-diphenol:dioxygen oxidoreductases), chosen as catalysts for dioxygen reduction. The laccases were immobilized in a liquid crystalline cubic phase formed with monoolein. The structures of the cubic phases, both with and without enzymes, were established using small-angle X-ray scattering. The catalytic reduction of dioxygen was performed using a glassy carbon electrode modified with cubic phases containing the enzymes. The modified electrode was used as a dioxygen sensing system, based on the increasing reduction current of a suitable electrochemical probe in the presence of dioxygen.
一种酶催化过程已被用于监测双氧。所使用的酶是两种不同的漆酶（对二苯酚：双氧氧化还原酶），被选作双氧还原的催化剂。漆酶被固定在由单油酸甘油酯形成的液晶立方相中。利用小角X射线散射确定了有无酶时立方相的结构。使用含有酶的立方相修饰的玻碳电极进行双氧的催化还原。基于在双氧存在下合适的电化学探针还原电流的增加，该修饰电极被用作双氧传感系统。
