Nazaruk Ewa, Bilewicz Renata, Lindblom Göran, Lindholm-Sethson Britta
Faculty of Chemistry, University of Warsaw, ul. Pasteura 1, 02093, Warsaw, Poland.
Anal Bioanal Chem. 2008 Jul;391(5):1569-78. doi: 10.1007/s00216-008-2149-y. Epub 2008 May 17.
Incorporation of membrane proteins with retained activity in artificial membranes for use in membrane-based sensors has attracted scientists for decades. This review briefly summarises general concepts on relevant cubic phases with and without incorporated proteins and provides some insight into the development of biosensors where bicontinuous cubic phases are used for incorporation of an enzyme. Some new data on impedance characterisation of a supported cubic phase are also shown. An efficient membrane-based electrochemical biosensor requires that the analyte has free access to the immobilised membrane protein and that regeneration of the catalysing enzyme is fast. Long-term stability of the system is also necessary for the biosensor to find applications outside the research laboratory. These basic concepts are discussed in the review along with presentation of those biosensing systems based on cubic phases that are reported in the literature.
几十年来，将具有保留活性的膜蛋白整合到用于基于膜的传感器的人工膜中一直吸引着科学家。本文简要总结了有关含或不含整合蛋白的相关立方相的一般概念，并深入探讨了使用双连续立方相整合酶的生物传感器的发展情况。还展示了一些关于支撑立方相阻抗表征的新数据。一种高效的基于膜的电化学生物传感器要求分析物能够自由接触固定化的膜蛋白，并且催化酶的再生要快。该系统的长期稳定性对于生物传感器在研究实验室之外找到应用也是必要的。本文在回顾中讨论了这些基本概念，并介绍了文献中报道的基于立方相的生物传感系统。
