Li Hanying, Park Sung Ha, Reif John H, LaBean Thomas H, Yan Hao
Department of Computer Science, Duke University, Durham, North Carolina 27708, USA.
J Am Chem Soc. 2004 Jan 21;126(2):418-9. doi: 10.1021/ja0383367.
Self-assembling DNA tiling lattices represent a versatile system for nanoscale construction. Self-assembled DNA arrays provide an excellent template for spatially positioning other molecules with increased relative precision and programmability. Here we report an experiment using a linear array of DNA triple crossover tiles to controllably template the self-assembly of single-layer or double-layer linear arrays of streptavidin molecules and streptavidin-conjugated nanogold particles through biotin-streptavidin interaction. The organization of streptavidin and its conjugated gold nanoparticles into periodic arrays was visualized by atomic force microscopy and scanning electron microscopy.
自组装DNA平铺晶格代表了一种用于纳米级构建的通用系统。自组装DNA阵列提供了一个出色的模板，用于以更高的相对精度和可编程性在空间上定位其他分子。在此，我们报告了一项实验，该实验使用DNA三向交叉瓦片的线性阵列，通过生物素-链霉亲和素相互作用，可控地模板化链霉亲和素分子和链霉亲和素共轭纳米金颗粒的单层或双层线性阵列的自组装。通过原子力显微镜和扫描电子显微镜观察到链霉亲和素及其共轭金纳米颗粒组织成周期性阵列。
