Harada N
Division of Molecular Genetics, School of Medicine, Fujita Health University, Aichi, Japan.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):1001-7. doi: 10.1016/0006-291x(92)92303-f.
Aromatase mRNA in human skin fibroblasts was greatly increased in the presence of dexamethasone. Aromatase cDNA was prepared depending on mRNA of dexamethasone-treated fibroblasts by the PCR method including the rapid amplification of cDNA ends (RACE) protocol. The isolated cDNA had the same sequence as placental aromatase cDNA in the region encoded by exons 2-10. However, all 5'-fragments obtained from fibroblast aromatase cDNA contained a unique sequence in the region encoded by exon 1. A unique sequence was also deduced for the region between exons 1 and 2 of the placental aromatase gene from its cDNA, indicating that this region is used as an exon 1 by alternative splicing in skin fibroblasts. Tissue-specific use of multiple exons 1 in the splicing of aromatase transcripts was demonstrated by the mRNAs obtained from various tissues.
在存在地塞米松的情况下，人皮肤成纤维细胞中的芳香化酶mRNA大幅增加。根据经地塞米松处理的成纤维细胞的mRNA，通过包括cDNA末端快速扩增（RACE）方案的PCR方法制备芳香化酶cDNA。分离得到的cDNA在由外显子2 - 10编码的区域与胎盘芳香化酶cDNA具有相同序列。然而，从成纤维细胞芳香化酶cDNA获得的所有5'-片段在由外显子1编码的区域都包含一个独特序列。从胎盘芳香化酶基因的cDNA中也推断出外显子1和2之间区域的独特序列，表明该区域在皮肤成纤维细胞中通过可变剪接被用作外显子1。从各种组织获得的mRNA证明了在芳香化酶转录本剪接中多个外显子1的组织特异性使用。
