Sharonov B P, Churilova I V
Institute of Pure Biopreparations, St. Petersburg, Russia.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):1129-35. doi: 10.1016/0006-291x(92)92321-n.
Bovine superoxide dismutase (SOD) was inactivated during incubation with phorbol myristate acetate-stimulated neutrophils. In addition, stimulated neutrophils were able to disrupt the SOD structure. Inactivation and structural damage were dependent on the action of hypochlorous acid, an oxidant generated by the myeloperoxidase-hydrogen peroxide-chloride system of neutrophils. Incubation of SOD with stimulated neutrophils lead to long-wavelength fluorescence (ex, 350 nm; em, 450 nm) and the appearance of new structural forms with other isoelectric points. These additional forms possess catalytic activity. Generation of catalytically active new forms of SOD demonstrates the inaccessibility of the active centre of SOD to hypochlorite and may be a reason for the successful application of SOD during anti-inflammatory therapy.
牛超氧化物歧化酶（SOD）在与佛波酯肉豆蔻酸酯乙酸盐刺激的中性粒细胞孵育期间失活。此外，受刺激的中性粒细胞能够破坏SOD的结构。失活和结构损伤取决于次氯酸的作用，次氯酸是由中性粒细胞的髓过氧化物酶 - 过氧化氢 - 氯化物系统产生的一种氧化剂。SOD与受刺激的中性粒细胞孵育会导致长波长荧光（激发波长，350nm；发射波长，450nm）以及出现具有其他等电点的新结构形式。这些额外的形式具有催化活性。产生具有催化活性的新形式的SOD表明SOD的活性中心对次氯酸盐具有不可及性，这可能是SOD在抗炎治疗中成功应用的一个原因。
