Kochman M L, DelValle J, Dickinson C J, Boland C R
Department of Internal Medicine, VA Medical Center, Ann Arbor, MI.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):1165-9. doi: 10.1016/0006-291x(92)92326-s.
Gastrin has been postulated to stimulate proliferation in colorectal neoplasms. Although gastrin mRNA has been demonstrated to be present in colon cancer cell lines, the intact peptide had not been recovered from human colorectal neoplasms. We demonstrate that gastrin and its precursors are present in both colorectal neoplasia and adjacent normal-appearing colonic mucosa. In colonic tissue, the glycine-extended precursor form of the peptide is over 10-fold more abundant than the amidated gastrin, and progastrin is more than 700-fold more abundant. In contrast, amidated gastrin in the human antrum is the predominant form of gastrin by a factor of 10. Furthermore, the ratio of gastrin precursors to gastrin is significantly increased in neoplastic colonic mucosa when compared with normal colonic tissue. These data suggest that the processing of gastrin is unique in the human colon and that further differences in processing occur in neoplastic colonic tissue.
胃泌素被认为可刺激结肠肿瘤的增殖。尽管已证实在结肠癌细胞系中存在胃泌素信使核糖核酸，但尚未从人类结肠肿瘤中分离出完整的肽。我们证明胃泌素及其前体存在于结肠肿瘤和相邻外观正常的结肠黏膜中。在结肠组织中，该肽的甘氨酸延伸前体形式比酰胺化胃泌素丰富10倍以上，而胃泌素原则比酰胺化胃泌素丰富700倍以上。相比之下，人胃窦中的酰胺化胃泌素是胃泌素的主要形式，其含量是其他形式的10倍。此外，与正常结肠组织相比，肿瘤性结肠黏膜中胃泌素前体与胃泌素的比例显著增加。这些数据表明，胃泌素的加工在人类结肠中是独特的，并且在肿瘤性结肠组织中加工存在进一步差异。
