Kinoshita Shigeru, Nakamura Takahiro
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Artif Organs. 2004 Jan;28(1):22-7. doi: 10.1111/j.1525-1594.2004.07319.x.
Regenerative medicine via tissue engineering is a newly developed medical field based on the use of somatic stem cells to generate biological substitutes and improve tissue functions. The achievement of these objectives depends on two important factors: stem cells, which are high in proliferability and differentiability, and the substrates that support them. In order to reconstruct the ocular surface in patients with severe ocular surface diseases, we decided to investigate the feasibility of human amniotic membrane as an epithelial carrier, and found that denuded amniotic membrane was the most appropriate substrate for this purpose. To develop the surgical treatment using denuded amniotic membrane as a carrier, we established the tissue engineering system for making transplantable epithelial sheets of either corneal or oral mucosal epithelial cells for ocular surface reconstruction.
通过组织工程进行的再生医学是一个新发展起来的医学领域，它基于利用体干细胞来生成生物替代品并改善组织功能。实现这些目标取决于两个重要因素：具有高增殖能力和分化能力的干细胞，以及支持它们的基质。为了重建严重眼表疾病患者的眼表，我们决定研究人羊膜作为上皮载体的可行性，并发现脱细胞羊膜是实现这一目的最合适基质。为了开发以脱细胞羊膜为载体的手术治疗方法，我们建立了组织工程系统，用于制备可移植的角膜或口腔黏膜上皮细胞片以进行眼表重建。
