Joner Erik J, Hirmann Doris, Szolar Oliver H J, Todorovic Dragana, Leyval Corinne, Loibner Andreas P
LIMOS (Laboratoire des Interactions Microorganismes-Minéraux-Matière Organique dans les Sols)-CNRS UMR 7131, Henri Poincaré University, Faculty of Science, PO Box 239, F-54506 Vandoeuvre-les-Nancy Cedex, France.
Environ Pollut. 2004;128(3):429-35. doi: 10.1016/j.envpol.2003.09.005.
An experiment was conducted to distinguish priming effects from the effects of phytoremediation of a creosote-polluted soil. The concentration of 13 polycyclic aromatic hydrocarbons (PAHs), and their combined soil toxicity (using four bioassays), was determined on recently excavated, homogenized soil and on such soil subjected to a time-course phytoremediation experiment with lucerne. The results showed a high priming effect, with minor positive and synergistic effects of planting and fertilization on PAH degradation rates. At the end of the experiment, PAH degradation reached 86% of the initial 519 mg PAHs kg(-1). Two of the four toxicity tests (bioluminescence inhibition and ostracod growth inhibition) corroborated the chemical data for residual PAHs, and indicated a significant reduction in soil toxicity. We conclude that priming effects can easily surpass treatment effects, and that an unintentional pre-incubation that ignores these effects can jeopardize the full quantitative assessment of in situ bioremediation of contaminated soil.
进行了一项实验，以区分在对受杂酚油污染的土壤进行植物修复过程中引发效应与植物修复效应。在最近挖掘并均质化的土壤以及进行了紫花苜蓿随时间进程植物修复实验的此类土壤上，测定了13种多环芳烃（PAH）的浓度及其综合土壤毒性（使用四种生物测定法）。结果显示出较高的引发效应，种植和施肥对PAH降解率有轻微的正向和协同效应。在实验结束时，PAH降解达到初始519 mg PAHs kg(-1)的86%。四种毒性测试中的两项（生物发光抑制和介形虫生长抑制）证实了残留PAH的化学数据，并表明土壤毒性显著降低。我们得出结论，引发效应很容易超过处理效应，并且忽略这些效应的无意预培养可能会危及对污染土壤原位生物修复的全面定量评估。
