Zamaeva N Iu, Antonov I V, Misharin A Iu
Bioorg Khim. 1992 Sep;18(9):1221-8.
A phosphatidyl choline (PC) exchange between apoHDL/PC micellar complexes in solution and the same complexes immobilized on Sepharose was studied. The PC exchange in buffer was represented in terms of pseudo first order reversible process. The first order constants for the unidirectional efflux of PC from apoHDL/PC-Sepharose (k1) and for the unidirectional efflux of PC from apoHDL/PC complexes (k2) were equal to (0.45 +/- 0.2) x 10(-3) and (1.35 +/- 0.2) x 10(-3) min-1, respectively. The k1 values showed the Arrhenius dependence on the temperature within range 278-323 K. Plasma serum proteins facilitated the PC efflux from apoHDL/PC-Sepharose being additional acceptors of PC. These data allow use of apoHDL/PC-Sepharose for correcting lipid plasma composition in vitro.
研究了溶液中载脂蛋白高密度脂蛋白/磷脂酰胆碱（apoHDL/PC）胶束复合物与固定在琼脂糖上的相同复合物之间的磷脂酰胆碱（PC）交换。缓冲液中的PC交换以伪一级可逆过程表示。PC从apoHDL/PC-琼脂糖单向流出的一级常数（k1）和PC从apoHDL/PC复合物单向流出的一级常数（k2）分别等于（0.45±0.2）×10⁻³和（1.35±0.2）×10⁻³ min⁻¹。k1值在278 - 323 K范围内显示出对温度的阿伦尼乌斯依赖性。血浆血清蛋白促进了PC从apoHDL/PC-琼脂糖的流出，成为PC的额外受体。这些数据允许在体外使用apoHDL/PC-琼脂糖来校正血脂成分。
