Antonov I V, Zamaeva N Iu, Medvedeva N V, Misharin A Iu
Bioorg Khim. 1992 Sep;18(9):1214-20.
There was developed a procedure for immobilization of phosphatidyl cholines (Egg yolk phosphatidyl choline and polyunsaturated soya beams phosphatidyl choline) on the modified apoHDL-Sepharose. The formation of phospholipid micelles was proved by linear dependence of the content of the sorbed phosphatidyl choline versus, the content of apoHDL bound to Sepharose. Incubation of apoHDL/PC-Sepharose with human plasma was shown to change the plasma lipid composition. The apoHDL/PC-Sepharose might be used for correction of the plasma lipid composition on vitro experiments.
已开发出一种将磷脂酰胆碱（蛋黄磷脂酰胆碱和多不饱和大豆磷脂酰胆碱）固定在修饰的载脂蛋白HDL-琼脂糖上的方法。通过吸附的磷脂酰胆碱含量与结合到琼脂糖上的载脂蛋白HDL含量的线性关系证明了磷脂胶束的形成。载脂蛋白HDL/PC-琼脂糖与人血浆孵育可改变血浆脂质组成。载脂蛋白HDL/PC-琼脂糖可用于体外实验中血浆脂质组成的校正。
