Gao J, Zeng B X, Zhou L J, Yuan S Y
Department of Anesthesiology, Union, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
Br J Anaesth. 2004 Feb;92(2):277-9. doi: 10.1093/bja/aeh050.
To investigate the effects of propofol administration on acute lung injury in endotoxin-induced shock in rats.
Seventy-six male Wistar rats were randomly assigned to one of five groups: (i) saline control; (ii) endotoxin alone (receiving lipopolysaccharide (LPS) 8 mg kg(-1) i.v.); (iii) pretreatment with propofol 1 h before LPS; (iv) simultaneous treatment with propofol and LPS; (v) post-treatment with propofol 1 h after LPS. During the 5 h after LPS injection, survival rates were recorded. Lung tissue was sampled to measure values of nitrite/nitrates (NO(2-)/NO(3-)) and tumour necrosis factor (TNF)-alpha in bronchoalveolar lavage (BAL) fluid, and wet-to-dry lung weight ratio, pulmonary permeability index, BAL protein and expression of inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT).
Compared with the endotoxaemic group, both the pre- and simultaneous treatment groups showed significantly improved 5 h survival rates, and attenuated endotoxin-induced increased BAL fluid NO(2-) /NO(3-) and TNF-alpha, iNOS mRNA and NT expression in lung tissue, and decreased pulmonary microvascular permeability. These beneficial effects were blunted in the post-treatment group.
These findings indicate that early administration of propofol may provide protective effects against endotoxin-induced acute lung injury.
研究丙泊酚给药对大鼠内毒素诱导性休克所致急性肺损伤的影响。
76只雄性Wistar大鼠随机分为五组之一：（i）生理盐水对照组；（ii）单独注射内毒素（静脉注射脂多糖（LPS）8 mg kg⁻¹）；（iii）在注射LPS前1小时用丙泊酚预处理；（iv）丙泊酚与LPS同时给药；（v）在注射LPS后1小时用丙泊酚后处理。在注射LPS后的5小时内，记录存活率。采集肺组织样本，以测定支气管肺泡灌洗（BAL）液中亚硝酸盐/硝酸盐（NO₂⁻/NO₃⁻）和肿瘤坏死因子（TNF）-α的值，以及肺湿重与干重之比、肺通透性指数、BAL蛋白以及诱导型一氧化氮合酶（iNOS）和硝基酪氨酸（NT）的表达。
与内毒素血症组相比，预处理组和同时给药组的5小时存活率均显著提高，内毒素诱导的BAL液中NO₂⁻/NO₃⁻和TNF-α增加、肺组织中iNOS mRNA和NT表达以及肺微血管通透性降低均得到减轻。后处理组的这些有益作用减弱。
这些发现表明，早期给予丙泊酚可能对内毒素诱导的急性肺损伤具有保护作用。
