Gao Ju, Zeng Bangxiong, Zhou Luojing
Department of Anesthesiology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.
Middle East J Anaesthesiol. 2003 Oct;17(3):379-401.
To investigate the effects of treatment with propofol administration at different time point in acute lung injury of endotoxin-induced shock rats.
76 male wistar rats were randomly assigned to five groups: A) control group; B) endotoxemic group, receiving intravenous lipopolysaccharide (LPS) 8 mg.kg-1; C) pretreatment group, treated identically to endotoxemic group with the additional administration of propofol (5 mg.kg-1 bolus, followed by infusion at 10 mg.kg-1.h-1) of 1 hr prior to the injection of LPS; D) simultaneously treatment group, treated identically to endotoxemic group with the additional administration of propofol simultaneously with the injection of LPS; E) post-treatment group, which was treated identically to endotoxemic group except for administration of propofol 1 hr after the injection of LPS. PaO2, pH, MAP and survival rate were recorded and plasma NO, TNF-alpha were measured during 5-hr after the injection of LPS. After the rats were killed, lung tissue was sampled to measured expression of inducible nitric oxide synthase (iNOS), nitrotyrosine (NT), myeloperoxidase (MPO) activity, malondialdehyde (MDA), wet-to-dry lung weight ratio (W/D), and pulmonary permeability index (PPI).
Compared with the endotoxemic group, both the pretreatment and simultaneously treatment groups, significantly improved PaO2, pH, MAP and 5th hour survival rate of rats, and attenuated endotoxin-induced increased iNOSmRNA, NT expression, MPO activity and MDA level in lung tissue, and decreased pulmonary microvascular permeability, TNF-alpha, NO in plasma. But these beneficial efficacies were blunted in the post-treatment group.
These findings showed that propofol administration may provide protective effects on acute lung injury in endotoxin-induced shock.
研究不同时间点给予丙泊酚对内毒素诱导休克大鼠急性肺损伤的治疗效果。
76只雄性Wistar大鼠随机分为五组：A）对照组；B）内毒素血症组，静脉注射脂多糖（LPS）8 mg·kg-1；C）预处理组，与内毒素血症组处理相同，但在注射LPS前1小时额外给予丙泊酚（5 mg·kg-1推注，随后以10 mg·kg-1·h-1输注）；D）同时治疗组，与内毒素血症组处理相同，但在注射LPS的同时额外给予丙泊酚；E）治疗后组，除在注射LPS后1小时给予丙泊酚外，其余处理与内毒素血症组相同。记录注射LPS后5小时内的PaO2、pH、平均动脉压（MAP）和生存率，并检测血浆一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）。大鼠处死后，取肺组织检测诱导型一氧化氮合酶（iNOS）、硝基酪氨酸（NT）的表达、髓过氧化物酶（MPO）活性、丙二醛（MDA）、肺湿重与干重比（W/D）及肺通透性指数（PPI）。
与内毒素血症组相比，预处理组和同时治疗组均显著改善了大鼠的PaO2、pH、MAP及5小时生存率，减轻了内毒素诱导的肺组织iNOSmRNA、NT表达、MPO活性及MDA水平升高，降低了肺微血管通透性、血浆TNF-α及NO水平。但这些有益作用在治疗后组减弱。
这些研究结果表明，给予丙泊酚可能对内毒素诱导休克的急性肺损伤具有保护作用。
