Smetana Karel, Mericka Otakar, Saeland Sem, Homolka Jirí, Brabec Jirí, Gabius Hans-Joachim
Institute of Anatomy, Charles University, 1st Faculty of Medicine, Unemocnice 3, 128 00, Prague 2, Czech Republic.
Virchows Arch. 2004 Feb;444(2):171-4. doi: 10.1007/s00428-003-0952-6. Epub 2004 Jan 13.
The diagnosis of pulmonary Langerhans cell histiocytosis might be refined by demonstrating reliability of a new cell marker, i.e., Langerin (CD207), used on bronchoalveolar lavage fluid. For this purpose, we collected material from patients with this disease and also with sarcoidosis and idiopathic pulmonary fibrosis as controls. In addition to the immunocytochemical detection of Langerin, we examined the expression profiles of CD1a and the macrophage tandem-repeat mannose receptor (CD206). To test accessibility of Langerin, a C-type lectin, for mannosides, we employed reverse lectin histochemistry using mannose-containing neoglycoproteins. The analysis revealed a significantly increased percentage of CD1a- and Langerin-positive cells in pulmonary Langerhans cell histiocytosis in comparison with both other studied diseases. No expression of the 175-kDa mannose-binding lectin (CD206) in Langerhans cells was observed. Evidently, binding sites on the cells were not accessible for the mannose-containing neoglycoligand. These results provide evidence for the usefulness of Langerin-directed immuno- and glycohistochemical monitoring of bronchoalveolar lavage fluid in the diagnosis of pulmonary Langerhans cell histiocytosis.
通过证明一种新的细胞标志物，即朗格素（CD207）用于支气管肺泡灌洗液体的可靠性，肺朗格汉斯细胞组织细胞增多症的诊断可能会更加精确。为此，我们收集了患有这种疾病的患者以及作为对照的结节病和特发性肺纤维化患者的样本。除了对朗格素进行免疫细胞化学检测外，我们还检测了CD1a和巨噬细胞串联重复甘露糖受体（CD206）的表达谱。为了测试作为C型凝集素的朗格素对甘露糖苷的可及性，我们使用含甘露糖的新糖蛋白进行了反向凝集素组织化学。分析显示，与其他两种研究疾病相比，肺朗格汉斯细胞组织细胞增多症中CD1a和朗格素阳性细胞的百分比显著增加。未观察到朗格汉斯细胞中175-kDa甘露糖结合凝集素（CD206）的表达。显然，含甘露糖的新糖配体无法与细胞上的结合位点结合。这些结果为朗格素导向的支气管肺泡灌洗液体免疫和糖组织化学监测在肺朗格汉斯细胞组织细胞增多症诊断中的有用性提供了证据。
