Estepa A, Frías D, Coll J M
INIA-Sanidad Animal, Valdeolmos, Madrid, Spain.
Viral Immunol. 1992 Winter;5(4):283-92. doi: 10.1089/vim.1992.5.283.
Viral hemorrhagic septicemia virus (VHSV) lysed the macrophages from rainbow trout kidney cultures either isolated by plastic adherence or stimulated with purified glycoprotein G from VHSV. The trout macrophages supported the replication of VHSV as tested by cell culture and by sandwich ELISA of the supernatants from infected cultures. VHSV-infected macrophages showed a decrease in both acridine-orange fluorescence and average size. Immunofluorescence studies with flow cytometry showed positive membrane staining with monoclonal antibodies (MAbs) anti-N and anti-G VHSV. These findings open the possibility of using trout macrophages as presenting cells to study the possible existence of helper or cytotoxic epitopes relevant to the protection of trout against VHSV.
病毒性出血性败血症病毒（VHSV）可裂解从虹鳟鱼肾脏培养物中分离得到的巨噬细胞，这些巨噬细胞要么通过塑料贴壁法分离，要么用来自VHSV的纯化糖蛋白G刺激。如通过细胞培养以及对受感染培养物上清液进行夹心酶联免疫吸附测定（ELISA）所检测的那样，虹鳟鱼巨噬细胞支持VHSV的复制。受VHSV感染的巨噬细胞在吖啶橙荧光和平均大小方面均有所降低。采用流式细胞术进行的免疫荧光研究显示，抗VHSV N和抗VHSV G单克隆抗体（MAbs）可使细胞膜呈阳性染色。这些发现开启了利用虹鳟鱼巨噬细胞作为呈递细胞来研究与保护虹鳟鱼抵御VHSV相关的辅助或细胞毒性表位可能存在情况的可能性。
