Langhans Bettina, Schweitzer Susann, Nischalke Hans Dieter, Braunschweiger Ingrid, Sauerbruch Tilman, Spengler Ulrich
Department of Internal Medicine I, University of Bonn, Bonn, Germany.
J Infect Dis. 2004 Jan 15;189(2):248-53. doi: 10.1086/380763. Epub 2004 Jan 8.
We studied the induction of T cell responses against hepatitis C virus (HCV)-related cytotoxic T cell (CTL) epitopes in lymphocytes from human leukocyte antigen (HLA)-A2-positive, HCV-naive individuals. Peptide and lipopeptides corresponding to the epitope at amino acids (aa) 1073-1081 induced equivalent numbers of tetramer-positive CD8+ T cells and detectable interferon-gamma and granzyme B spot-forming cells (sfcs). Epitope aa 35-44 induction of CTLs was not achieved, despite detectable tetramer-positive CD8+ T cells. IFN-gamma sfcs could only be induced with lipopeptide aa 20-44 but not by conventional antigens, which indicates that lipopeptides may alter T cell functions, depending on the epitope.
我们研究了在人类白细胞抗原（HLA）-A2阳性、未感染丙型肝炎病毒（HCV）的个体的淋巴细胞中，针对HCV相关细胞毒性T细胞（CTL）表位的T细胞应答的诱导情况。与氨基酸（aa）1073 - 1081处表位对应的肽和脂肽诱导出数量相当的四聚体阳性CD8 + T细胞以及可检测到的干扰素-γ和颗粒酶B斑点形成细胞（sfcs）。尽管检测到四聚体阳性CD8 + T细胞，但未实现表位aa 35 - 44对CTL的诱导。干扰素-γ sfcs只能由脂肽aa 20 - 44诱导产生，而不能由传统抗原诱导产生，这表明脂肽可能会根据表位改变T细胞功能。
