一种由栖居于狗牙根（Cynodon dactylon）中的内生真菌——尖顶孢霉属（Calcarisporium sp.）产生的高效饲料防腐剂候选物。

A potent feed preservative candidate produced by Calcarisporium sp., an endophyte residing in stargrass (Cynodon dactylon).

作者信息

Ji L L, Song Y C, Tan R X

机构信息

Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.

出版信息

J Appl Microbiol. 2004;96(2):352-8. doi: 10.1046/j.1365-2672.2004.02157.x.

DOI:10.1046/j.1365-2672.2004.02157.x

PMID:14723696

Abstract

AIMS

The cultures of an endophytic fungus Calcarisporium sp. were screened for inhibitors on the growth of feed-associated moulds and on the aflatoxin biosynthesis to find a safe and effective feed preservative.

METHODS AND RESULTS

Eight test fungi were isolated from the spoiled poultry feed. The endophytic fungus Calcarisporium sp. was separated from the Chinese coastal grass Cynodon dactylon. The antifungal action concerning the endophytic culture extract (ECE) was performed with propionic acid (PPA) as the corresponding reference. The ECE had a similar antifungal efficacy to PPA in a concentration-dependent manner. The susceptibility order of the ECE to the test fungi was found to be Fusarium sp. > Aspergillus spp. > Penicillium spp. Furthermore, the application of the ECE in pelleted-layer duck feed as a preservative was carried out at a humidity of 10, 15 and 20%. It has been discerned that mould growth and aflatoxin biosynthesis could be co-inhibited almost completely by ECE at concentrations higher than 1.0% (w/w). The LD50 of the ECE on mice was shown to be higher than 28 g kg-1.

CONCLUSIONS

The ECE can be selected as an inhibitor to preserve poultry feed on inhibiting the growth of mould and aflatoxin biosynthesis during feed storage.

SIGNIFICANCE AND IMPACT OF THE STUDY

The ECE may be an effective and biosafe antifungal ingredient for poultry feed and holds a potential market prospect in feed industry.

摘要

目的

对一种内生真菌Calcarisporium sp.的培养物进行筛选，以寻找对饲料相关霉菌生长和黄曲霉毒素生物合成具有抑制作用的物质，从而找到一种安全有效的饲料防腐剂。

方法与结果

从变质家禽饲料中分离出8种测试真菌。内生真菌Calcarisporium sp.是从中国沿海草皮狗牙根中分离得到的。以丙酸（PPA）作为对照，对内生真菌培养物提取物（ECE）的抗真菌作用进行了研究。ECE具有与PPA相似的抗真菌效果，且呈浓度依赖性。发现ECE对测试真菌的敏感性顺序为镰刀菌属>曲霉属>青霉属。此外，在湿度为10%、15%和20%的条件下，将ECE作为防腐剂应用于蛋鸭颗粒饲料中。已发现，当浓度高于1.0%（w/w）时，ECE几乎可以完全共同抑制霉菌生长和黄曲霉毒素生物合成。ECE对小鼠的半数致死量（LD50）高于28 g kg-1。