促甲状腺激素受体在甲状腺相关性眼病患者眼外肌中的原位表达。
In situ expression of thyrotropin receptor in the extraocular muscles of patients with thyroid-associated ophthalmopathy.
作者信息
Chen Jing, Wei Rui-li, He Jin, Ma Xiao-ye
机构信息
Department of Ophthalmology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2004 Jan;24(1):7-10.
OBJECTIVE
To investigate the expression of thyrotropin receptor (TSHR) in the extraocular muscles (EOM) of the eyes of patients with thyroid-associated ophthalmopathy (TAO).
METHODS
EOM specimens were obtained from 12 patients with severe TAO and 10 healthy subjects within 1 hour after accidental death and the paraffin-embedded sections were observed by streptavidin-peroxidase (SP) immunohistochemical methods.
RESULT
The expression of TSHR was observed in the perimysium, cytomembrane and cytoplasm in 10 of the 12 TAO specimens, accounting for a TSHR expression rate of 83.3% in the EOM of TAO patients. No TSHR protein was found in the healthy subjects.
CONCLUSION
High TSHR expression in the EOM of TAO patients indicates that as a common antigen between the orbit and thyroid, TSHR play a key role in the pathogenesis of TAO.
目的
探讨促甲状腺激素受体（TSHR）在甲状腺相关性眼病（TAO）患者眼外肌（EOM）中的表达。
方法
在意外死亡后1小时内，从12例重度TAO患者和10例健康受试者获取EOM标本，采用链霉亲和素-过氧化物酶（SP）免疫组织化学方法观察石蜡包埋切片。
结果
12例TAO标本中有10例在肌束膜、细胞膜和细胞质中观察到TSHR表达，TAO患者EOM中TSHR表达率为83.3%。健康受试者未发现TSHR蛋白。
结论
TAO患者EOM中TSHR高表达表明，TSHR作为眼眶和甲状腺之间的共同抗原，在TAO发病机制中起关键作用。
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