Liu Xian, Xu Yi-kai, Huang Qi-liu
Medical Imaging Center, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Jan;24(1):15-7.
To explore the optimal reaction condition of DTPA-coupled streptavidin labeled with gadolinium ion.
The number streptavidin-coupled of gadolinium ions and maximum biotin-binding capacity of Gd-DTPA-SA complex were analyzed at different DTPA-to-SA molar ratios (1000, 500, 200, 100) and in buffer solutions of different pH values (pH 5, 6, 7, and 8, respectively).
The number of streptavidin-coupled gadolinium ions increased with the pH value of the reaction system. When the DTPA-to-SA molar ratio was below 500, the number of gadolinium ions that SA-DTPA coupled increased with the DTPA-to-SA molar ratio, but tended to decrease when the ratio was 1,000. No significant difference in the maximum biotin-binding capacity of Gd-DTPA-SA complex was noted between the groups.
The number of gadolinium ions that streptavidin coupled depends on the pH value of the reaction system and DTPA-to-SA molar ratio, but the maximum biotin-binding capacity of Gd-DTPA-SA complex is not affected by the reaction conditions.
